通过ATR-MIR光谱学通过体液样本对结直肠癌诊断的生物光谱方法与多变量数据分析相结合
Fuzuli Tugrul1, Gonul Akin Geyik2, Berrin Yalinbaş Kaya3
1Eskişehir City Hospital, Clinic of Radiation Oncology, 26080 Eskisehir, Turkey.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|September 9, 2023
概括
这项研究引入了一种使用ATR-MIR光谱的快速生物谱法,用于检测各种体液中的结直肠癌 (CC) 生物标志物. 该方法准确地区分了CC和健康样本,有助于早期诊断.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 癌症生物标志物 癌症生物标志物
- 分子诊断学 分子诊断学
背景情况:
- 结肠直肠癌 (CC) 的诊断依赖于侵入性手术.
- 对非侵入性,快速诊断工具的需求至关重要.
- 体液中的生物分子变化可以表明CC的存在.
研究的目的:
- 开发和验证用于CC检测的生物光谱方法.
- 在不同的体液中识别可表明CC的光谱生物标志物.
- 为了评估CC分类的多变量分析的有效性.
主要方法:
- 使用了减弱的全反射中红外光谱 (ATR-MIR).
- 分析了来自血,血清,唾液和洗液的200个样本.
- 应用多变量数据分析,包括HCA,LDA,PCA和SIMCA.
主要成果:
- 在CC病例中观察到脂质,蛋白质,核酸和碳水化合物度的显著变化.
- SIMCA 展示了优秀的分类性能.
- 线性差异分析 (LDA) 实现了所有标本的100%准确分类.
结论:
- ATR-MIR光谱学提供了一种快速而准确的方法,用于从体液中进行CC诊断.
- 这种技术可以作为人口查和预诊断决策的宝贵工具.
- 开发的方法支持非侵入性结肠直肠癌查.
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