评估长期使用的手洗手剂在皮肤上的抗菌疗效
K Duggan1, K Bentley2, R J Stanton2
1School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.
The Journal of hospital infection
|September 9, 2023
概括
一个ex-vivo猪皮模型有效地测量了无酒精手消毒剂的长期杀菌效果,为持续抗菌活性要求提供了传统测试方法的实用替代方案.
科学领域:
- 微生物学 微生物学
- 皮肤病学 皮肤病学
- 传染性疾病 传染性疾病
背景情况:
- 标准定量悬浮剂和指板测试对手部消毒剂的有效性在评估持久配方时是有限的.
- 这些传统方法面临挑战,包括生物风险,高成本和时间限制.
- 越来越多的长期抗微生物活性要求需要替代,实际的测试策略.
研究的目的:
- 调查标准化ex-vivo猪皮模型对长期有效性的可重复性评估的有用性.
- 用这个新型模型来评估一种无酒精手洗手剂配方.
主要方法:
- 用量化悬浮试验 (EN13727,EN14476) 用5分钟接触时间评估了对黄金葡萄球菌,大肠杆菌,肺炎菌和SARS-CoV-2的微生物杀菌疗效.
- 经过修改的PAS 2424协议在6小时的时间内在活体猪皮上使用,以模拟磨损并测量持续的疗效.
- 无酒精的洗手液是测试配方.
主要成果:
- 定量悬浮试验表明,在5分钟内,所有测试生物体的减少>5 log10 .
- 活体猪皮肤模型在所有时间点都显示出一致的,尽管降低的,微生物杀伤疗效.
- 皮肤磨损在猪皮肤模型中没有显著影响消毒剂的有效性.
结论:
- 活体猪皮模型提供了一个可行的和方便的系统来评估手洗剂配方的长期有效性.
- 该模型支持开发和证实关于洁手剂在皮肤上的作用的可持续性声明.
- 它为评估具有长时间抗微生物作用的配方提供了一个切实可行的替代方案.
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