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在Clamydomonas reinhardtii藻类中取决于物种的加多的摄取
Karolin Sommer1, Sarah Reuter2, Matthias Elinkmann1
1Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
The Science of the total environment
|September 9, 2023
概括
藻类吸收的基于加多的对比剂 (GBCA) 取决于特定的GBCA. 线性GBCA被藻类细胞吸收,与宏循环GBCA不同,引发了对食物链积累的担忧.
科学领域:
- 环境化学环境化学
- 生物地质化学生物地质化学
- 毒理学 毒理学 毒理学
背景情况:
- 基于加多的对比剂 (GBCA) 广泛用于医学成像.
- GBCAs的环境命运和生物吸收尚未完全理解.
- 藻类是主要生产者,可以成为污染物进入食物链的途径.
研究的目的:
- 为了研究从各种基于的对比剂 (GBCA) 吸收 (Gd) 到藻类细胞 (Chlamydomonas reinhardtii) 的物种依赖性吸收.
- 为了确定化后藻类细胞内和周围介质中的Gd的物种化.
- 评估GBCAs在水生生物中的生物积累方面的潜在环境风险.
主要方法:
- 用GdCl3和四种不同的GBCA (线性和宏循环) 化克拉米多马纳斯reinhardtii24小时.
- 使用单细胞诱导合血质谱法 (sc-ICP-MS) 分析细胞内Gd.
- 使用ICP-MS量化总Gd,使用水友互动液色谱 (HILIC) -ICP-MS分析细胞溶解物中的可溶性Gd的特异分析.
主要成果:
- 观察到Gd的取决于物种的吸收,线性GBCA和GdCl3显示显著的细胞吸收,而宏环GBCA显示最小的吸收.
- sc-ICP-MS检测到GdCl3和线性GBCA的细胞内Gd信号,但没有检测到宏循环GBCA.
- 在细胞溶解物中没有检测到完整的GBCA,这表明藻类细胞内的复杂物种变化或降解.
结论:
- 藻类对Gd的吸收和物种化高度依赖于GBCA的化学形式.
- 线性GBCA被藻类细胞吸收,并经历物种变化,表明潜在的环境风险.
- 需要进一步的研究,以确定酸盐中确切的Gd物种,并评估Gd在食物链中的积累潜力.
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