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通过miR-183/SMAD4轴,CircBTBD7可以抑制脂肪生成

Zheng Ma¹, Yun Chen¹, Ju Qiu¹

¹College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.

International journal of biological macromolecules

|September 9, 2023

概括

这项研究表明,circBTBD7抑制了奶牛的脂肪生成 (脂肪细胞形成). 它通过调节miR-183/SMAD4通路而起作用,为脂肪发育提供了新的见解.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
动物科学动物科学

背景情况:

循环RNAs (circRNAs) 通过脂肪生成的调节还没有完全被理解.
circRNAs在各种生物过程中起着至关重要的作用.
在牛脂肪生成中研究新型circRNA是必不可少的.

研究的目的:

为了识别和描述参与牛脂肪生成的新型circRNAs.
阐明circBTBD7在脂肪细胞分化中的调节机制.
探索受circBTBD7.7影响的分子途径.

主要方法:

在牛脂肪组织和分化脂肪细胞中识别和表达circRNAs的分析.
使用siRNA评估circBTBD7功能的 Knockdown实验.
定量实时PCR和西式涂抹测量基因和蛋白质表达.
双露西法酶记者测定证实了circRNA和miRNA之间的直接相互作用.
救援实验以验证监管轴.

主要成果:

一种新型的circRNA,circBTBD7,被发现,在牛脂肪组织和脂肪细胞分化过程中表达高.
抑制circBTBD7显著增加了脂质滴积累,并上调了关键的脂肪和脂质基因 (PPARγ,C/EBPα,FABP4,FASN,ACCα).
circBTBD7通过菌miR-183作为竞争的内源RNA (ceRNA) 起作用,从而减轻SMAD4的抑制,从而抑制脂肪生成.

结论:

circBTBD7在牛脂肪生成中起着抑制作用.
这种机制涉及circBTBD7,作为miR-183的ceRNA,它准SMAD4.
这种circRNA-miRNA-mRNA轴 (circBTBD7 / miR-183 / SMAD4) 为脂肪生成提供了一个新的调节途径.

关键词:

增脂生殖是一种增脂的过程.在SMAD4中,我们可以看到SMAD4.circBTBD7 circBTBD7 circBTBD7 circBTBD7 circBTBD7 circBTBD7 在 miR-183 系统中,

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