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威斯普1对帕拉克瓦特诱导的EMT的影响
Jingyan Liu1, Shengnan Lv2, Wanling Ma3
1Department of Emergency, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Longgang District People's Hospital of Shenzhen, Shenzhen, Guangdong 518172, PR China.
概括
温特诱导信号蛋白-1 (WISP1) 在帕拉奎特诱导的上皮层-介质细胞过渡 (EMT) 中起着关键作用. 抑制WISP1可以防止EMT,为帕奎特中毒提供潜在的治疗点.
科学领域:
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
- 肺部医学 肺部医学
背景情况:
- 暴露于帕拉克瓦特 (PQ) 是肺纤维化 (PF) 的已知原因之一.
- 在膜上皮细胞中,PQ通过促进上皮-介质细胞过渡 (EMT) 来诱导PF.
- 在PQ诱导的EMT背后的精确分子机制仍然不清楚.
研究的目的:
- 研究Wnt诱导信号蛋白-1 (WISP1) 在PQ诱导的EMT中的作用.
- 为了确定WISP1是否是PQ中毒的潜在治疗标.
主要方法:
- 用PQ的不同度和持续时间对待A549细胞.
- 评估了细胞形态,细胞亡和死亡率.
- 使用qRT-PCR和西部斑块量化WISP1,E-cadherin和Vimentin表达水平.
- 为了评估其在PQ引起的EMT中的作用,WISP1被沉默了.
主要成果:
- 暴露于PQ改变了A549细胞形态,增加了细胞亡和死亡率,降低了E-cadherin,并提高了WISP1和Vimentin水平.
- 随着PQ治疗时间,WISP1基因表达显著增加.
- 沉默WISP1取消了PQ对E-cadherin和Vimentin的影响,并抑制了PQ诱导的EMT和形态变化.
结论:
- WISP1是膜上皮细胞中PQ诱导的EMT的关键媒介.
- 低调WISP1有效抑制PQ诱导的EMT.
- 威斯普1代表了一种有前途的治疗点,用于治疗帕拉奎特中毒.
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