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罗萨文通过HDAC1-诱导的EEF2表观遗传调节来调节骨质稳定
Wenhao Zhang1, Leilei Yu1, Fang Wang1
1Department of Oral and Craniofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China.
罗迪奥拉罗莎 (Rhodiola rosea) 是一种红植物.
科学领域:
- 药理学和分子生物学
- 骨生物学和疾病
背景情况:
- 罗迪奥拉L.含有具有多种药理作用的生物活性化合物.
- 罗萨文在骨质生成和骨质疏松症 (OP) 中的作用尚未完全理解.
- 骨质疏松症是一种重要的骨疾病,其特点是骨质损失.
研究的目的:
- 研究罗萨文对卵巢切除术 (OVX) 诱导的小鼠骨质疏松症的治疗作用.
- 阐明罗萨文在骨质平衡中的作用背后的分子机制.
主要方法:
- 在小鼠中的卵巢切除 (OVX) 模型来诱导骨质疏松症.
- 在体外测定骨质细胞和骨质细胞活力的测试.
- 转录组测序,基因本体学 (GO) 丰富分析和蛋白质-蛋白质相互作用 (PPI) 网络构建.
- 基因素脱乙烯化试验和救援实验.
- 对NF-κB和MAPK信号通路的分析.
主要成果:
- 罗萨文在OVX诱导的骨质疏松症上表现出治疗作用,抑制骨质细胞活力,促进骨质细胞活力.
- 确定了HDAC1/EEF2轴作为罗萨文作用的关键分子通路.
- HDAC1通过激素脱乙烯化抑制了EEF2的表达;EEF2逆转了HDAC1对骨质平衡的影响.
- 罗萨抑制了NF-κB和MAPK通路,其作用由HDAC1/EEF2轴调节.
结论:
- 罗萨文通过调节EEF2.2的基因素乙化来维持骨质疏松症中的骨质稳定性.
- 罗萨文显示出作为治疗骨质疏松症治疗的治疗候选人的潜力.
- HDAC1/EEF2轴是Rosavin骨保护作用的关键目标.
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