相关实验视频

Updated: Jul 16, 2025

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

微RNA作为绝经前骨质疏松症的潜在生物预测剂:生物化学和分子研究

Hadeel A Al-Rawaf¹, Sami A Gabr², Amir Iqbal³

¹Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, 11433, Saudi Arabia.

BMC women's health

|September 9, 2023

概括

循环中的microRNAs (miRNAs) 在绝经前妇女骨质疏松症的非侵入性生物标志物中表现有前途. 发现特定的miRNA与骨密度和传统的骨健康标志物相关.

科学领域:

生物化学生物化学
分子生物学分子生物学
内分泌学在内分泌学.

背景情况:

循环微RNA (miRNA) 是骨健康的新兴生物标志物.
它们在血清等体液中的测量提供了可行性和简单性.

研究的目的:

在绝经前妇女中评估特定的miRNA表达 (miR-21,miR-24,miR-100,miR-24a,miR-103-3p,miR-142-3p).
评估这些miRNA与骨健康标志物 (骨素,性酸酶,脱氧氨) 之间的相关性.

主要方法:

实时聚合酶连锁反应 (RT-PCR) 用于miRNA表达分析.
血清生物标志物的免疫检测 (骨素,s-BAP,DPD).
定量超声波 (QUS) 和DXA扫描用于骨矿物密度评估.

主要成果:

在骨质疏松症和骨质疏松症中,miR-21,miR-24和mir-100被上调.
在骨质疏松症中,miR-24a,miR-103-3p和miR-142-3p的下调.
上调和下调的miRNA与骨矿物质密度和血清骨标记物有显著的相关性.

结论:

循环中的miRNA与已确定的骨健康参数相关.
这些miRNAs可能作为在绝经前妇女骨质疏松症的非侵入性生物标志物.
建议对更大的样本进行进一步研究,以验证其诊断潜力.

关键词:

这就是为什么B.M.D. BMD.微RNAs是一种微RNA.骨质疏松症是一种骨质疏松症.在QUSUS中,你会看到QUS.血清骨标记物血清骨标记物 c-BUAUA 的意思是

更多相关视频

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...