在Salmonella enterica serovar Typhi中对curli (csg) 基因表达的差异调控需要的不仅仅是功能性的CsgD调控器
Camille Ou1,2, Charles M Dozois2,3, France Daigle4,5
1Department of Microbiology, Infectiology and Immunology, University of Montréal, 2900 Bd Édouard-Montpetit, Montreal, QC, H3T 1J4, Canada.
Scientific reports
|September 9, 2023
概括
沙门氏菌Typhi不会产生形膜,这是其他沙门氏菌血清体中必不可少的生物膜成分. 这表明S. Typhi的独特调控变化,独立于卷曲基因群,控制卷曲的生产.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 沙门氏菌 (Salmonella enterica serovar Typhi) (S. Typhi) 是导致伤寒的疾病之一.
- 形膜是沙门氏菌中的关键生物膜成分,由csg基因集群编码.
- 尽管有证据表明它们在感染期间产生的证据,但S. Typhi中曲线的功能仍然不清楚.
研究的目的:
- 为了研究在S. Typhi.的曲线基因表达.
- 确定S. Typhi的曲线阴性表型是由于csg基因集群或其调节的差异引起的.
主要方法:
- 分析了S Typhi CsgD蛋白与csgB促进体的结合.
- 用S Typhimurium等位基因替换了S Typhi csgD基因.
- 将整个S Typhimurium csg基因集群克隆成S Typhi.
主要成果:
- S. Typhi CsgD 蛋白质对 csgB 促进体具有较弱的结合.
- 将S. Typhi csgD与S. Typhimurium等位基因替换并没有恢复curli的产生.
- 引入功能性S. Typhimuriumcsg基因集群并没有导致S. Typhi.中可检测到的曲线.
结论:
- S. Typhi无法产生曲线不仅仅是由于CsgD调节器的差异.
- 不同的调节机制,独立于csg基因集群,可能会控制S. Typhi与其他沙门氏菌血清型相比,在S. Typhi中控制curli基因表达.
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