在沙门氏菌中发现了两种不同调节的长体的证据
Sónia Castanheira1, Francisco García-Del Portillo2
1Laboratory of Intracellular Bacterial Pathogens, National Centre for Biotechnology (CNB)-CSIC, Darwin 3, 28049, Madrid, Spain.
Communications biology
|September 9, 2023
概括
细菌可以有两个不同的细胞形状决定系统,长相,这使得像沙门氏菌这样的病原体能够适应不同的环境. 这一发现挑战了以前对细菌形态学的理解.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 细菌病原体的产生
背景情况:
- 细菌细胞形状是基因决定的,对于利基适应至关重要.
- 棒状细菌利用两种主要系统,延长体和分裂体,用于细胞壁合成.
- 对于微生物来说,不管它们的环境如何,一般都假定细胞形态不变.
研究的目的:
- 调查病原体沙门氏菌中细胞形状的决定背后的机制.
- 探索单一细菌物种内多个形态遗传系统的存在.
- 了解细菌在不同的环境条件下如何保持形状.
主要方法:
- 清理不同的长相复合体.
- 对环境暗示反应的分析.
- 识别特定的糖甘合成酶 (PBP2和PBP2SAL),指导每个延长体.
- 长相运动速度的比较分析.
主要成果:
- 在沙门氏菌中,有两个功能延长体的证据,每一个都负责保持棒形状.
- 每个延长体都由不同的甘氨酸合成酶 (PBP2和PBP2SAL) 指导.
- 在中性pH下,PBP2-elongasome的功能很好,而PBP2SAL-elongasome在酸性条件下运作,运动速度更慢.
- 在其他病原体中存在替代性形态遗传系统.
结论:
- 沙门氏菌采用两种不同的长相体,以在不同的宿主环境中保持其形状.
- 病原体可能拥有替代系统来维持形态,挑战了适应利基的形态可塑性概念.
- 这一发现对了解细菌适应和病原体中的毒性有意义.
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