转录基因分析揭示了基于VI型分泌系统抑制的Cronobacter的新型干燥耐受性机制
Yang Wang1, Na Ling1, Rui Jiao1
1School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.
Food research international (Ottawa, Ont.)
|September 10, 2023
概括
克罗诺巴克特 (Cronobacter malonaticus) 通过减少非必不可少的功能和抑制VI型分泌系统 (T6SS) 来在干旱条件下生存. 这种T6SS抑制是其干燥耐受性在婴儿配方奶粉等食品中的关键.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 分子生物学分子生物学
背景情况:
- 克罗诺巴克特 (Cronobacter malonaticus) 是一种通过食物传播的病原体,会引起严重的感染.
- 它可以在干粉婴儿配方 (PIF) 中长期生存,表明强烈的干燥耐受性.
- 这种抗性背后的分子机制尚未完全理解.
研究的目的:
- 为了阐明Cronobacter malonaticus干燥抵抗的分子机制.
- 调查VI型分泌系统 (T6SS) 在干燥耐受性中的作用.
主要方法:
- 在干燥条件下对C. malonaticus进行转录基因分析 (7,15,30天).
- 在干燥后对野生类型和T6SS突变物生存率的比较分析.
- 在其他克罗诺细菌物种中检查T6SS基因表达.
主要成果:
- 干燥引发了普遍的适应策略:增加了兼容溶液,激活了压力调节剂,抑制了蛋白质出口和分泌系统,并减少了毒性因子.
- 第六类分泌系统 (T6SS) 基因被显著下调.
- 干燥后T6SS突变体显著增加了生存和生存能力,这表明T6SS对干燥耐受性有负面调节.
结论:
- 克罗诺巴克特菌 spp. 的种类. 具有一种涉及T6SS抑制的新型抗干燥机制.
- 抑制T6SS可降低宏分子分泌,并防止细胞膜高度,提高干燥过程中的生存率.
- 这一发现对于了解和预防干燥食品中的Cronobacter污染至关重要.
更多相关视频
12:18A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
10.3K
08:11Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris
Published on: January 3, 2025
487
相关概念视频
Transcription Attenuation in Prokaryotes
15.4K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.4K
Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
Gene Regulation During Sporulation
34
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
34
CRISPR and crRNAs
17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
Other Stress Responses in Bacteria
33
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33
Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K
