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微生物群落及其与在机械化生产淡香味Baijiu的过程中形成风味化合物的相关性
Lu-Jun Luo1, Liang Song1, Ying Han2
1State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Food research international (Ottawa, Ont.)
|September 10, 2023
概括
机械化轻味的Baijiu发酵涉及明显的微生物转移. 在这个工业过程中,关键的细菌如Bacillus和Lactobacillus,以及诸如Saccharomyces cerevisiae之类的真菌,驱动着风味化合物的形成.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 发酵技术的发酵技术
背景情况:
- 淡味的Baijiu发酵传统上依赖于各种微生物的自发固态发酵.
- 越来越多的机械化在Baijiu生产需要了解潜在的微生物和物理化学动力学.
- 机械化过程中微生物群体的继承和风味的发展并没有得到很好的描述.
研究的目的:
- 为了研究在机械化轻味Baijiu发酵过程中微生物社区的继承.
- 确定与机械化Baijiu生产中风味化合物形成相关的关键微生物.
- 了解微生物相互作用及其对发酵过程的影响.
主要方法:
- 使用常规稀释涂层进行微生物计数.
- 使用PacBio单分子实时 (SMRT) 测序用于微生物社区概况.
- 应用头部空间固态微提取与气色谱质谱学 (HS-SPME-GC-MS) 结合,用于风味化合物分析.
主要成果:
- 在浸泡和发酵阶段观察到不同的真菌和细菌群体.
- 在主要的细菌 (例如,Pantoea agglomerans到Bacillus物种,Lactobacillus acetotolerans) 和真菌 (例如,Cladosporium exasperatum到Saccharomyces cerevisiae,Lichtheimia ramosa) 物种中发现了显著的继承.
- 特定的微生物物种与增加的酸度,乙醇生产和各种风味化合物相关,并确定了共发生和共排除模式.
结论:
- 与传统方法相比,机械发酵显著改变了微生物社区的动态.
- 确定了关键的细菌和真菌物种在机械化Baijiu发酵过程中在风味的发展中起着至关重要的作用.
- 结果为优化机械发酵过程提供了洞察力,以改善轻味的Baijiu生产.
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