对模拟敏感的Cdk1作为研究线性退出的工具:在发芽酵母中,蛋白质酸酶1是从Cdk1无活化下游所需的
Jason M Keaton1,2, Benjamin G Workman2, Linfeng Xie2
1Acacia Safety Consulting, Inc, P.O. Box 342603, Milwaukee, WI, 53234, USA.
概括
蛋白质激酶Cdk1 / 环素B的特定无活化会触发酵母中的线性退出. 这一发现表明,Cdk1的不活化足以启动细胞周期从线粒分裂转移到G1阶段的过渡.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 在Saccharomyces cerevisiae中,线性退出需要对循环素依赖的激酶Cdk1/循环素B (Cdc28/Clb2) 的失活.
- 了解线粒体退出的精确触发因素和下游事件对于理解细胞循环调节至关重要.
研究的目的:
- 调查Cdk1/cyclin B的特异性无活化是否足以启动发芽酵母从线粒分裂中退出.
- 建立一个测试基因要求的系统,在线粒体退出过程中对Cdk1无活化的下游测试.
主要方法:
- 利用对1NM-PP1敏感的cdc28-as1突变酵母菌株,专门抑制Cdk1 (Cdc28).
- 在用1NM-PP1.1治疗之前,细胞在线粒分裂中被使用子毒物阻止.
- 监测的细胞事件表明G1进入,包括重新发芽,交配投影形成,Sic1稳定和Clb2降解.
- 采用结合cdc28-as1与条件突变的遗传方法来评估基因需求.
主要成果:
- 在cdc28-as1细胞中使用1NM-PP1对Cdk1/cyclin B (Cdc28/Clb2) 的特异性抑制引发了快速的脱离线分裂.
- 细胞启动了G1阶段的事件,如重新芽生长和shmoo形成,而没有完成细胞运动.
- 证明Cdk1无活化不仅是必要的,而且足以启动从线粒分裂过渡到G1.
- 鉴定了蛋白酸酶1 (PPase1; Glc7) 作为 mitotic 退出和 Cdk1 失活后互相重建的必需物.
结论:
- 在Saccharomyces cerevisiae中,Cdk1/cyclin B无活化是启动Saccharomyces cerevisiae从线粒分裂过渡到G1阶段的足够触发因素.
- 开发的cdc28-as1系统为剖析cdk1失活的下游途径提供了一个强大的工具.
- 蛋白酸酶1 (PPase1) 是一种关键的调节器,需要在Cdk1失活后进行线粒体退出和相间恢复.
关键词:
1NM-PP1 1NM-PP1 1NM-PP1 1NM-PP1 1NM-PP1 1NMCdc28 在线阅读在Cdk1/cyclin B中使用.细胞循环的细胞周期在MPF的MPF.线粒分裂 (mitosis) 是一种发生在细胞的过程.米托斯出口的出口.芽的复苏 复发的复苏cdc28-as1 在线阅读更多相关视频
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