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在芽酵母中,形长链基和醇的同时结构更换

Yushi Kono¹, Yohei Ishibashi², Shizuka Fukuda¹

¹Department of Chemistry, Faculty of Sciences, Kyushu University, Fukuoka, Japan.

The FEBS journal

|September 10, 2023

概括

酵母细胞被改造为具有哺乳动物脂蛋白 (sphingosine) 而不是本地细胞 (phytosphingosine) 显示生长缺陷和应激敏感性. 用哺乳动物类型取代脂和固醇结构并没有解决这些问题.

科学领域:

* 细胞和分子生物学
* 生物化学 * 生物化学
* 酵母遗传学公司

背景情况:

*生物膜表现出特定物种的脂质组成,特别是哺乳动物和酵母 (Saccharomyces cerevisiae) 之间的长链基 (LCB) 和固醇的差异.
* 哺乳动物利用斯芬戈辛作为LCB脊柱,用于胺和脂,以及胆固醇作为它们的主要类固醇.
* 酵母相反,使用植物素作为LCB和厄戈斯特醇作为固醇;酵母不能自然合成素.

研究的目的:

* 用于描述被改造为合成斯芬戈的酵母细胞,而不是合成植物素的酵母细胞 (sphingosine细胞).
* 为了研究酵母LCB用sphingosine替代的代谢要求和表型后果.
* 建立和分析酵母细胞,同时用哺乳动物对应物 (sphingosine/胆固醇细胞) 替换LCB和固醇结构.

主要方法:

*产生缺乏内源性LCB生物合成的酵母菌株,并补充了外源性LCBs.
* 仅含斯芬戈的酵母细胞的表征,包括在受阻胺/斯芬戈脂生物合成条件下的生长试验.
* 酵母菌株的开发和分析,同时用哺乳动物类型替换LCB和固醇结构.

主要成果:

*当胺或复杂脂生物合成被抑制时,胺酵母细胞表现出显著的生长缺陷,这表明需要进一步的胺代谢.
*这些状细胞对各种环境压力表现出过敏,并表现出异常的血膜和细胞壁特性.
*同时用哺乳动物结构 (神素/胆固醇细胞) 替换LCB和固醇并没有挽救观察到的压力过敏和膜/细胞壁异常.

结论:

* 酵母细胞可以通过斯芬戈替代植物素生存下来,但这需要进一步对斯芬戈进行代谢处理.
*即使与哺乳动物固醇相结合,斯芬戈辛的存在也会导致酵母细胞的完整性和抗压能力受到损害.
*这项研究首次证明,酵母可以被改造为包含哺乳动物的LCB和固醇结构,尽管导致显著的表型变化.

关键词:

这种植物是Saccharomyces cerevisiae.长链基础的长链基础球脂质是指脂质中的一种.这是一种固醇 (sterol) 固醇.压力耐受性承受压力耐受性

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