商用大豆蛋白分离物的不同来源:与蛋白质功能相关的结构,组成和物理化学特征
Longteng Zhang1, Qian Li2, Wei Zhang3
1Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; Department of Food Science, University of Copenhagen, 1958 Frederiksberg C, Denmark.
Food chemistry
|September 10, 2023
概括
商用大豆蛋白分离物 (SPI) 在质量上有很大的差异. 加工引起的变化,而不是子单元组成,主要决定了大豆蛋白的功能,如溶解性和结合能力.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 大豆蛋白分离物 (SPI) 是广泛使用的食品成分.
- 了解SPI特性和功能之间的联系对于食品应用至关重要.
- 商业SPI质量的变化可能会影响产品的一致性.
研究的目的:
- 研究商业SPI的物理化学性质,亚单元组成和蛋白质功能之间的关系.
- 根据加工诱导的修改对SPI进行分类.
- 确定影响SPI功能的主要因素.
主要方法:
- 分析来自中国和欧盟的商业SPI.
- 物理化学性质的表征,包括变性,疏水性和颗粒大小.
- 评估蛋白质功能,如水结合能力 (WBC),油结合能力 (OBC) 和可溶性.
- 评估甘氨酸/β-凝聚甘氨酸亚单元的组成.
主要成果:
- 观察到SPI组成,变性,WBC,OBC和溶解度的显著变化.
- SPI分为预先化,部分化和较少修饰的组.
- 预先变质的SPI显示由于聚合而导致结合能力降低.
- 部分化SPI表现出更高的溶解度从暴露的水友性区域和减少的分子大小.
- 处理引起的变化比子单位组成对功能更有影响.
结论:
- 商业SPI原材料的质量差异很大.
- 处理显著改变SPI的物理化学性质和功能.
- 基于加工修改的分类方法对SPI来说是有希望的.
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