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基于α-螺旋的抑制剂的设计和评估,以阻止PD-1/PD-L1相互作用

Mengjie Rui¹, Wen Zhang¹, Ke Mi¹

¹Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, PR China.

International journal of biological macromolecules

|September 10, 2023

概括

研究人员开发了新的α螺旋类来阻止PD-1/PD-L1相互作用,增强抗瘤免疫力. 改性可以作为癌症免疫治疗的稳定检查点抑制剂.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
生物化学生物化学

背景情况:

阻止PD-1/PD-L1蛋白与蛋白相互作用 (PPI) 是瘤免疫疗法的关键策略.
阿尔法螺旋结构对于蛋白质相互作用至关重要,可以通过来模拟以调节PPI.

研究的目的:

设计和评估以α螺旋为基础的类作为PD-1/PD-L1相互作用的抑制剂.
评估这些的治疗潜力,以恢复抗瘤免疫活性.

主要方法:

基于已知的蛋白质结构的α螺旋的设计.
使用分子对接和动力学模拟进行选.
在体外和体内验证,包括HTRF,SPR,共同培养和动物模型.

主要成果:

两种候选物有效抑制PD-1/PD-L1相互作用,并恢复了抗瘤免疫力.
D-氨基酸替代增强了血清稳定性,并促进了静脉注射.
开发的体显示出显著的抗瘤作用,并改善了T细胞免疫力.

结论:

基于α螺旋的体设计是开发蛋白质-蛋白质相互作用抑制剂的可行策略.
开发的具有作为癌症免疫疗法的新检查点抑制剂的潜力.
这种方法可以扩展到设计其他关键PPI的抑制剂.

关键词:

这是ApoA-1模仿.癌症免疫疗法癌症免疫疗法在PD-1/PD-L1检查站.类抑制剂类抑制剂虚拟选是一个虚拟的选.

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