通过pH转移增强的修饰肝蛋白上的基结构依赖相互作用:蛋白质界面和乳化性质的调节
Yangyang Hu1, Changyu Zhou1, Lihui Du1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food & Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
International journal of biological macromolecules
|September 10, 2023
概括
改性肝蛋白 (MGLP) 乳化特性被多醇增强,特别是丁. 这些MGLP-多复合物提供了改进的乳液稳定性和抗氧化性.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 体科学 体科学 体科学
背景情况:
- 与肉类蛋白相比,动物副产品蛋白作为乳化剂的使用不足.
- 肝蛋白 (MGLP) 具有作为乳化剂的潜力,但其特性需要修改.
- 聚醇可以与蛋白质相互作用,改变它们的功能性质.
研究的目的:
- 研究结构不同的多 (甲基素,素,素) 对改性肝蛋白 (MGLP) 的界面和乳化特性的影响.
- 评估pH值转移对多-MGLP相互作用和随后的乳液稳定性的影响.
- 确定多化学结构在制造有效的MGLP-多复合物的作用,以稳定乳液.
主要方法:
- 使用六种多 (甲基素,素,鲁丁及其pH转移的对应物) 来修改MGLP.
- 使用热力学参数 (ΔH, ΔS) 和表面疏水性测量分析多-MGLP相互作用.
- 使用界面压力和膨胀弹性模量对界面性质的评估.
- 评估MGLP多稳定乳液的乳液稳定性 (物理和氧化) 和脂肪酸概况.
主要成果:
- 奎尔因与MGLP的水相互作用较强,而与甲基素和鲁丁相比,受其结构的影响.
- 聚烯相互作用亲和力与MGLP表面疏水性正相关,与MGLP大小和聚合性负相关.
- 协同的多相互作用和pH值转移显著增强了MGLP界面吸附和结合,特别是与P-quercetin.
- 由P-quercetin稳定的乳液表现出优越的物理稳定性 (较低的颗粒大小和度指数) 和氧化稳定性,保持更高的不和脂肪酸含量.
结论:
- 聚的化学结构是形成具有增强乳液稳定能力的MGLP-聚复合物的关键决定因素.
- 改变pH值的奎尔素 (P-奎尔素) 显著改善了MGLP的界面和乳化特性,导致更稳定的乳液.
- 这项研究突出了利用转基因动物副产品蛋白质具有特定多的潜力,用于先进的食品乳液应用.
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