基于糖糖的空间变化直径的血管网络通道的支架的准备
Siyu Chen1,2, Qianmin Gao3,4,5, Qingxi Hu1,2,6
1Rapid Manufacturing Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, People's Republic of China.
Biomedical materials (Bristol, England)
|September 11, 2023
概括
研究人员开发了一种新的生物脚手架制造方法,使用糖模板来创建复杂的血管网络,用于组织工程. 这种技术确保了用于大规模组织修复的优良细胞活力和机械性能.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 组织工程在创建具有复杂结构的大规模支架方面面临着挑战.
- 发展功能性血管网络对于工程组织中的营养和氧气运输至关重要.
研究的目的:
- 提出一种新的牺牲模板工艺,用于制造具有复杂,多层分支血管网络的生物支架.
- 为大规模组织修复验证制造生物支架的可行性,准确性和生物相容性.
主要方法:
- 使用了用糖和凝/微生物转胺酶溶液的牺牲模板工艺.
- 分析了形态特征,血管通道连接,成型精度和机械性能.
- 使用人类静脉内皮细胞 (HUVECs) 评估细胞的附着,生长和增殖.
- 在小鼠体内评估生物相容性和通过皮下植入进行组织再生.
主要成果:
- 制造的生物支架表现出完整的形态和出色的血管网络连接.
- 证明了糖模板直径和料速度之间的线性关系,直径误差<8%.
- 机械性能满足了大规模组织缺陷修复的要求.
- 在体外和体外的研究表明,细胞附着均,高活力,在血管通道内组织再生.
结论:
- 提出的牺牲模板方法成功地产生了适合组织工程的复杂血管网络的生物支架.
- 制造的生物支架显示出出色的生物相容性和机械完整性,用于大规模的组织缺陷修复.
- 这种方法为工程组织中氧气和营养物质的运输提供了一个有希望的新策略,具有潜在的临床应用.
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