鉴定和描述与蚊子微生物组相关的两个CRISPR/Cas系统
Shivanand Hegde1,2, Hallie E Rauch3, Grant L Hughes1
1Department of Vector Biology and Tropical Disease Biology, Liverpool School of Tropical Medicine, Centre for Neglected Tropical Disease, Liverpool, UK.
Access microbiology
|September 11, 2023
概括
研究人员在蚊子肠道细菌中确定了两种新型聚类定期间隔的短平行体重复 (CRISPR) /Cas系统. 这些系统,CRISPR1 (I-E型) 和CRISPR2 (I-F型),表现出不同的目标偏好,影响微生物相互作用.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 蚊子微生物组影响载体特征,为疾病控制策略提供了潜在的可能性.
- 了解宿主-微生物相互作用是开发针对蚊子传播疾病的新型基于微生物的方法的关键.
研究的目的:
- 在 *Serratia* sp. 中描述两种新型聚类定期间隔的短平行体重复 (CRISPR) / Cas 系统. Ag1,一种来自 *Anopheles gambiae* 蚊子肠的分离物.
- 研究这些已识别的CRISPR/Cas系统的表达和目标特异性.
主要方法:
- 孤立的 * 塞拉提亚 * sp. 来自 *Anopheles gambiae* 蚊子肠中的Ag1.
- 基于cas基因组合的两个不同的CRISPR/Cas系统 (CRISPR1:I-E型,CRISPR2:I-F型) 的识别和分类.
- 通过RT-PCR分析,确认在对数增长过程中的cas基因表达.
- 生物信息分析用于识别菌体和等离子体序列中的潜在间隔器目标.
主要成果:
- CRISPR1 (I-E型) 的目标主要是等离子体序列 (91%),而CRISPR2 (I-F型) 的目标主要是菌体基因组 (66%).
- 克里斯普尔2系统与其他*塞拉提亚*物种共享相同的直接重复序列,这表明水平基因转移.
- 两种系统中所有已识别的cas基因都在培养中的对数增长过程中得到表达.
结论:
- 这项研究强调了蚊子肠道共生体中CRISPR位点的存在和活性.
- 克里斯普尔1和克里斯普尔2的独特目标特异性表明,它们在蚊子肠道环境中的移动遗传元素管理中扮演着不同的角色.
- 这些发现表明,在蚊子中的细菌共生体和侵入性遗传元素之间,在进化过程中存在着复杂的相互作用.
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