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基于MicroTom番茄的基因组和RNAome的番茄器官发育的全面监管网络
Jia-Yu Xue1, Hai-Yun Fan1, Zhen Zeng2
1College of Horticulture, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.
Horticulture research
|September 11, 2023
概括
一种模型植物MicroTom提供了对器官发育和植物微生物相互作用的见解. 研究人员建立了基因共同表达网络,并确定了非编码RNA,为未来的研究创造了一个有价值的数据库.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 由于其短生长周期和高转化效率,MicroTom是一个有前途的模型工厂.
- 它适用于研究器官发育,新陈代谢和植物微生物相互作用.
- 一个新的参考基因组和广泛的RNA-seq数据可用于MicroTom.
研究的目的:
- 为 MicroTom.Tom 构建基因共同表达网络.
- 识别非编码RNAs (miRNAs, lncRNAs, circRNAs) 和它们的目标.
- 为MicroTom的研究建立一个全面的数据库.
主要方法:
- 组装一个参考基因组为MicroTom.
- 分析来自各种组织,发育阶段和治疗的RNA测序数据.
- 基因共同表达网络和非编码RNA相互作用网络 (miRNA-lncRNA,miRNA-mRNA,lncRNA-mRNA) 的构建.
主要成果:
- 构建了多个基因共同表达网络,提供了对调节途径的洞察.
- 确定了包括miRNA,lncRNA和circRNA在内的非编码RNA及其潜在的mRNA标.
- 建立了涉及非编码RNA和基因的交互网络.
- 创建了一个数据库 (http://eplant.njau.edu.cn/microTomBase/),集成基因组,转录基因组和网络数据.
结论:
- 这项研究为MicroTom.提供了宝贵的基因组和转录组资源.
- 构建的网络为识别植物生长,共生和水果发育中的关键基因提供了线索.
- 微数据库是研究人员利用这种模型植物的重要资源.
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