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赫斯佩雷丁对乳腺癌细胞MLH1和MSH2表达的作用BT-54949

Assim Khattab Hasan¹, Esmaeil Babaei¹, Ahmed Salim Kadhim Al-Khafaji²

¹Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

Journal of advanced pharmaceutical technology & research

|September 11, 2023

概括

一种天然的黄类化合物hesperetin (HSP) 通过选择性抑制癌细胞生长和诱导亡,显示出作为乳腺癌治疗的潜力. 这种表观遗传剂在乳腺癌细胞上表现出比正常细胞更强的细胞毒性作用,这表明它是一个有前途的治疗途径.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

乳腺癌的异质性带来了治疗挑战.
传统疗法有显著的副作用.
作为替代品,人们正在探索像黄类的天然化合物.

研究的目的:

研究hsp的细胞毒性和抗恶性潜力在乳腺癌细胞上.
检查HSP对基因表达和亡的影响.
为了比较HSP对乳腺癌细胞和正常细胞的疗效.

主要方法:

细胞毒性的MTT测定.
实时PCR用于基因表达分析 (MLH1,MSH2).
流细胞测量用于检测细胞亡.

主要成果:

对BT-549 (乳腺癌) 和MCF-10a (正常) 细胞,HSP表现出剂量依赖的细胞毒性,癌细胞的IC50较低.
在两个细胞系中观察到MLH1和MSH2基因的上调,在高度的HSP中.
高色素诱导了依赖度的细胞死亡和早期的细胞亡,特别是在乳腺癌细胞中.

结论:

赫斯佩雷丁对乳腺癌细胞具有选择性细胞毒性.
HSP诱导亡和调节基因表达的能力表明它具有治疗潜力.
进一步研究HSP作为乳腺癌的天然治疗剂是有必要的.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.BT 549 细胞的细胞在MIC50中,MIC50是MIC50.MLH1 一个问题在MSH2中,MSH2是MSH2.在MTT测试中使用MTT测试.乳腺癌乳腺癌乳腺癌流动细胞计量是流动细胞计量的方法.没有希望,没有希望.mRNA的表达方式

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