鉴定了一种致病原体,该致病原体是Sanghuangporus vaninii的果体腐烂
Weidong Yuan1, Lin Ma2, Xingkun Chen3
1Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
PeerJ
|September 11, 2023
概括
一种致病性真菌,Trichoderma virens,被确定为种植的Sanghuangporus vaninii果实体的疾病原因. 这一发现对于保护这种有价值的药用巨型真菌和防止行业经济损失至关重要.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 药用菌类学 药用菌类学
背景情况:
- 桑黄 (Sanghuangporus vaninii) 是一种药用宏观真菌,在中国具有重要的栽培价值.
- 培养S. vaninii受到致病真菌的威胁,造成了相当大的经济损失.
- 疾病症状在果实体发育的早期阶段表现出来.
研究的目的:
- 为了识别影响Sanghuangporus vaninii种植的致病性真菌.
- 为了验证分离的真菌的致病性.
- 评估已识别的病原体对S. vaninii的对抗潜力.
主要方法:
- 从患病的S. vaninii中分离出致病性真菌 (YZB-1).
- 通过注射S. vaninii果实体进行致病性测试.
- 隔离物的形态和分子识别 (rDNA ITS区域)
- 对于真菌生长的温度稳定性测试.
- 双培养试验用于评估对抗效应.
主要成果:
- 通过形态和分子分析,YZB-1分离物被证实是Trichoderma virens.
- 验证了病原性,因为YZB-1引起的疾病症状类似于在栽培中观察到的症状.
- 在25°C时,Trichoderma virens表现出最佳的细胞生长.
- 双培养试验显示,在25°C时,T. virens对S. vaninii菌根的抑制率很高 (79.01±2.79%).
结论:
- 特里科德尔马病毒被确定为影响桑胡安波鲁斯 (Sanghuangporus vaninii) 种植的主要病原体.
- 了解T. virens的最佳生长条件和对抗潜力对于疾病管理策略至关重要.
- 这项研究为保护S. vaninii的生产和减轻经济损失提供了关键的见解.
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