主要粉生物合成酶的起源和演变
Hong Chang1,2,3, Jie Bai1,2,3, Hejian Zhang1,2,3
1College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Synthetic and systems biotechnology
|September 11, 2023
概括
植物中粉生物合成的进化涉及横向基因转移和内共生. 诸如粉合成酶 (SS),粉分枝酶 (SBE) 和异氨酶 (ISA) 等关键酶起源于细菌,并在最后一个真核生物共同祖先 (LECA) 之前获得.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 植物科学 植物科学
背景情况:
- 粉是植物中重要的能量储存分子,对食品和工业应用至关重要.
- 粉生物合成途径酶的进化起源仍然不完全理解.
- 关键酶包括粉合成酶 (SS),粉分枝酶 (SBE) 和异酶 (ISA).
研究的目的:
- 研究粉生物合成途径酶的进化历史和起源.
- 为了阐明在真核生物中塑造粉生物合成途径的基因转移事件.
- 了解植物中SS,SBE和ISA酶的多样化.
主要方法:
- 对SS,SBE和ISA酶进行了全面的遗传学分析.
- 在51,151个注释的基因组中对这些酶的结构分析.
- 通过比较基因组学和祖先重建来追踪进化起源.
主要成果:
- SBE的祖先很可能起源于一个未知的细菌通过横向基因转移 (HGT) 在最后一个真核生物共同祖先 (LECA) 之前.
- 在Archaeplastida的出现过程中,从Deltaproteobacteria (HGT) 和Chlamydiae (内共生基因转移 - EGT) 获得了一个SS类和ISA.
- 随后的SS,SBE和ISA的重复和域修改导致了专门的异形和Viridiplantae的完整途径.
结论:
- 粉生物合成途径在真核生物中是多个基因获取事件的结果,包括HGT和EGT.
- 这些事件为早期的真核生物提供了碳水化合物代谢的基础酶机制.
- 这项研究揭示了植物生物学中的关键进化步骤和基本代谢途径的发展.
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