对于HIV-1和SARS-CoV-2包裹病毒的无活化而使用的阴阳基托桑衍生物
Zamani E D Cele1, William Matshe1, Lindani Mdlalose1
1Bio-Polymer Modification and Advanced Therapeutics Laboratory, Centre for Nanostructures and Advanced Materials, Council for Scientific and Industrial Research, Pretoria, Gauteng 0001, South Africa.
ACS omega
|September 11, 2023
概括
像HTCC这样的阴离子基托衍生物对包括HIV和SARS-CoV-2在内的包裹病毒表现出强大的抗病毒活性. 覆盖HTCC的口罩几乎消除了SARS-CoV-2,证明了实际应用的潜力.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 病毒学 病毒学
背景情况:
- 化基衍生物被探索为抗菌剂.
- 抗病毒活性和基托衍生物对包裹病毒的作用机制尚不清楚.
研究的目的:
- 评估基酸移植奇托 (HPA-CS) 和N-(2-基) -3-三甲基奇托化物 (HTCC) 对包裹病毒的抗病毒功效.
- 研究作用机制,重点关注膜破坏和病毒蛋白相互作用.
- 评估HTCC在抗病毒口罩中的实际应用.
主要方法:
- 血液溶解试验,以评估膜破坏潜力.
- 基于 luciferase 的抗病毒测定对抗HIV-1 NL4.3.3.
- 使用HTCC涂层和未涂层非医疗口罩进行SARS-CoV-2捕获测定.
- 分子对接以预测与病毒尖端蛋白的相互作用.
主要成果:
- 阴离子基托桑衍生物诱导了显著的血液溶解,表明膜破坏.
- 在HTCC中,针对HIV-1的抗病毒活性强 (IC50=0.225 mg/mL),表现优于HPA-CS (IC50=4.109 mg/mL).
- 覆盖HTCC的口罩实现了近100%的SARS-CoV-2抑制,而没有涂层的口罩则显示了不到60%的抑制.
结论:
- HTCC对HIV和SARS-CoV-2等包裹病毒具有显著的抗病毒特性.
- 该机制涉及膜破坏和与病毒尖端蛋白直接相互作用,防止细胞进入.
- 涂有HTCC的口罩为非医疗环境中增强病毒保护提供了有希望的解决方案.
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