Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

39.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.3K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

97.1K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
97.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Development of the Paraphilic Disorders Short Screen (PDSS) and Its Examination in Diverse Populations: A Large, Multi-Nation Study.

Sexual abuse : a journal of research and treatment·2026
Same author

The prevention of sexual violence in school settings with adolescents: A study protocol for the cluster randomized trial of the SUN (stand up now against sexual violence) program.

Contemporary clinical trials·2026
Same author

Correction: Penile prosthesis implantation: a systematic review of intraoperative and postoperative complications.

International journal of impotence research·2026
Same author

Fear of falling in older adults: The role of fear of dependency and tiredness as key determinants.

Geriatric nursing (New York, N.Y.)·2026
Same author

UltraFast Layer-Resolved Encoding (uFLARE) functional MRI deciphers bidirectional signaling from spontaneous activity.

Nature communications·2026
Same author

Bone Health in Former Artistic Gymnasts Aged 45 Years and Over: Case-Control Comparison with Controls and Reference Populations.

International journal of environmental research and public health·2026
Same journal

NMR Spectroscopy: Molecular Insights into Cell Wall Collapse and Oxidative Stress of <i>Escherichia coli</i> Induced by Imidazole-Activated Eutectic Solvents.

ACS omega·2026
Same journal

Enhanced Arsenite Remediation in Synthetic FeS<sub>2</sub>/Fe(II)-Containing Arsenic Wastewater via Epigallocatechin Gallate-Initiated Persulfate Activation.

ACS omega·2026
Same journal

Defect and Particle-Size Engineering as Mechanistic Drivers for Dye Uptake in a Zirconium Metal-Organic Framework.

ACS omega·2026
Same journal

Biogeochemical Assessment of Short-Term Hydrogen Storage in Methane Reservoirs with Field Sample Characterization and Reactor Experiments.

ACS omega·2026
Same journal

Combined Effects of Halloysite Nanotubes, Nucleating Agent, and Thermal Annealing on the Printability and Mechanical Performances of 3D-Printable Polypropylene Random Copolymer-Based Composites.

ACS omega·2026
Same journal

Effect of MoS<sub>2</sub> Interfacial Engineering across MAPbI<sub>3</sub>, FAPbI<sub>3</sub>, and CsPbI<sub>3</sub> Perovskite Solar Cells.

ACS omega·2026
查看所有相关文章

相关实验视频

Updated: Jul 16, 2025

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

25.9K

微型化微型固相提取基模块,用于从葡萄藤样本中高效地提取和净化DNA.

Carla Teixeira1,2,3, Andrey Ipatov1, Joana Carvalho1,4

  • 1International Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.

ACS omega
|September 11, 2023
PubMed
概括
此摘要是机器生成的。

研究人员使用微尺度固相提取 (μSPE) 开发了一种小型化DNA净化模块. 这种方法提供了一种从蔬菜样本中提取DNA的有效方法,改善了分散分析的DNA产量.

更多相关视频

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing
07:34

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing

Published on: December 1, 2023

759
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K

相关实验视频

Last Updated: Jul 16, 2025

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

25.9K
Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing
07:34

Author Spotlight: Exploring the Fermentation Microbiome Through Next-Generation Sequencing

Published on: December 1, 2023

759
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K

科学领域:

  • 生物技术是生物技术.
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 为了获得可靠的结果,DNA分析需要高效的提取和净化.
  • 微型化和降低成本是分散DNA分析的关键.
  • 微尺度固相提取 (μSPE) 显示出改善DNA产量的前景.

研究的目的:

  • 使用μSPE开发一个小型的DNA净化模块.
  • 为植物样本建立一个高效的DNA净化协议.
  • 与商业套件相比,评估开发的协议的性能.

主要方法:

  • 开发一个小型的μSPE模块,使用玻璃玻璃微纤维过器.
  • 建立植物样本 (叶子和葡萄) 的DNA净化协议.
  • 对DNA恢复产量的比较分析与商业DNA提取套件相比.

主要成果:

  • 开发的μSPE模块使得从各种植物基质中高效净化DNA成为可能.
  • 该协议显示,与商业套件相比,DNA回收率约为50%.
  • 该方法适用于分散的DNA分析应用.

结论:

  • 微型的μSPE模块和协议为从蔬菜样本中净化DNA提供了有效的解决方案.
  • 这种方法有助于简化,缩小和降低DNA分析的成本.
  • 开发的方法有可能在分散的遗传分析中得到更广泛的应用.