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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Published on: May 16, 2022

压缩超光谱显微镜用于癌症检测.

Yaniv Oiknine¹, Marwan Abuleil¹, Eugene Brozgol²

¹Ben-Gurion University of the Negev, School of Electrical and Computer Engineering, Electro-Optics and Photonics Engineering Department, Beer-Sheva, Israel.

Journal of biomedical optics

|September 11, 2023

概括

这项研究引入了一种使用压缩传感的快速超光谱显微镜系统,用于快速客观的癌症诊断. 该技术通过提供组织的详细光谱指纹来增强病理分析,有助于准确的预后.

关键词:

癌症检测癌症检测压力感应感应压力感应感应数字病理学数字病理学超光谱成像技术的使用.液晶调制器液晶调制器显微镜显微镜是指使用显微镜.

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科学领域:

生物医学光学生物医学光学
病理学病理学病理学
医疗成像医学成像

背景情况:

超光谱显微镜通过光谱指纹提供了详细的组织特征.
同时的分子探针分析有助于病理学家进行诊断和预后.
越来越多的病理学工作负载需要客观的工具来进行伴随性诊断和自动化癌症预后.

研究的目的:

开发一个快速,准确的超光谱显微镜系统,可以与现有显微镜集成.
为优化测量时间与光谱分辨率提供灵活性.
为改善癌症诊断和预后提供一个客观的工具.

主要方法:

使用压力传感器 (CS) 与光谱编码照明装置集成.
采用紧,快速运行的液晶电池进行光谱编码.
证明了用于快速超光谱图像采集的模块化和多功能性.

主要成果:

在不到3分钟内从乳腺癌活检中获得全幻灯片超光谱数据.
从47个编码图像中,重建了250个光谱带 (450-700 nm) 的超光谱图像.
在常见的血素和素染色活检上成功展示了CS超光谱显微镜.

结论:

CS高光谱显微镜对染色活检的标准实验室显微镜有效.
快速实现的高光谱分辨率支持苛刻的病理诊断.
该系统显示了改善癌症诊断和预后的潜力.