来自Bacillus licheniformis的热稳定细胞酶的特征 PANG L从喜马拉雅山土壤中分离出来
Manita Shyaula1,2, Sunil Regmi2, Deegendra Khadka2
1Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
International journal of microbiology
|September 11, 2023
概括
一种新的热友细菌Bacillus licheniformis PANG L从喜马拉雅土壤中分离出来. 这种细菌产生一种热稳定的细胞酶,具有工业应用的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- 热友细菌是工业过程中热稳定酶的宝贵来源.
- 细胞酶对于分解纤维素至关重要,在生物燃料生产,食品加工和织行业都有应用.
- 关于来自喜马拉雅山脉等独特地理位置的细胞酶产生热友细菌的研究有限.
研究的目的:
- 从喜马拉雅土壤中分离和描述一种热友细胞酶产生细菌.
- 为了优化细胞酶的生产条件.
- 为潜在的工业用途部分净化和确定分离的细胞酶的酶性质.
主要方法:
- 从喜马拉雅土壤中分离和选热友细菌.
- 使用形态,生理,生化和分子技术 (16S rRNA 测序) 识别有力的细胞酶生产物 (PANG L).
- 细胞酶生产参数的优化 (温度,pH,化时间,碳/来源,动).
- 通过硫酸沉和透析部分净化细胞质.
- 酶活性,稳定性,基质特异性和动力参数 (Km,Vmax) 的表征.
主要成果:
- 隔离了11种热友细菌;PANG L菌株显示出最高的细胞分解活性.
- 鉴定出PANG L菌株为一个名为*Bacillus licheniformis*的菌株.
- 在45°C,pH值为6.5的温度下,细胞质酶的最佳生成发生在48小时用碳氧甲基纤维素 (CMC) 和酵母提取物化后.
- 部分净化的细胞质在60°C和pH 5时表现出最佳活性,在酸性条件下 (pH 3-5),在高温下 (在55°C保持67%活性) 显示出稳定性.
- 酶活性被金属离子 (Cd2+,Pb2+,Ba2+) 增强,在CMC上表现出最高的活性.
- 确定了动力学参数:Km = 1.8 mg/ml和Vmax = 10.92 μg/ml/min. 这两个参数是:Km = 1.8 mg/ml和Vmax = 10.92 μg/ml/min.
结论:
- 这项研究成功地隔离并确定了一种来自喜马拉雅土壤的新型热友细胞酶产生*Bacillus licheniformis*菌株 (PANG L).
- 产生的细胞酶表现出热稳定和酸稳定性质,使其成为工业应用的有希望的候选者.
- 对这种酶的大规模生产和应用的进一步研究是有必要的.
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