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养后的转录学揭示了沙漠的中肠表达的基本基因

Joachim Van Lommel¹, Michiel Holtof¹, Laurentijn Tilleman²

¹Molecular Developmental Physiology and Signal Transduction Lab, Department of Biology, University of Leuven, Leuven, Belgium.

Frontiers in physiology

|September 11, 2023

概括

这项研究分析了食后沙漠虫中肠中的基因表达,揭示了关键的消化和免疫基因. 两种已识别的蛋白质是害虫控制策略的潜在目标.

科学领域:

昆虫生理学昆虫生理学
分子生物学分子生物学
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

消化道是动物的关键接口.
昆虫肠道转录组研究往往侧重于感染或异生菌,而不是消化.
在养后对基因表达的研究不足.

研究的目的:

在*Schistocerca gregaria*中,在养后的不同时间点比较中肠基因表达特征.
识别参与消化系统生理,免疫力和外来生物代谢的基因.
调查潜在的杀虫剂目标,以防治害虫.

主要方法:

对 *Schistocerca gregaria* 中肠的转录组分析.
基因表达在养后10分钟,2小时和24小时的比较.
RNA干扰 (RNAi) 用于评估基因功能和死亡率.

主要成果:

养2小时后576个基因的升级,与消化,免疫和异生物代谢有关.
*S. gregaria*中肠对食物存在的快速转录反应 (10分钟时间点).
已确定真空型H(+) -ATPase和尼曼-皮克1b蛋白是关键的,随着敲击增加死亡率.

结论:

*S. gregaria* 中肠表现出对食的动态转录反应.
空腔类型的H(+) -ATPase和Niemann-Pick 1b是重要的蛋白质和潜在的杀虫剂目标.
生成的转录组数据为研究这一重要害虫物种提供了资源.

关键词:

尼曼-皮克 1b 蛋白质在RNA-seqqq.沙漠的虫沙漠的虫消化消化消化消化昆虫是一种昆虫.在养后的养.转录组学转录组学是指转录组学.真空类型的H(+) -ATP酶

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