细胞骨激活NHE1调节机械敏感细胞体积适应和增殖
bioRxiv : the preprint server for biology
|September 11, 2023
概括
哺乳动物细胞使用细胞骨激活离子载体来调节细胞体积. 这一过程涉及流入和actomyosin重塑,驱动缓慢的二次体积增加 (SVI) 并影响基因表达.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 机械生物学 机械生物学
背景情况:
- 细胞体积调节对于哺乳动物细胞响应环境变化至关重要.
- 在细胞体积调节中,actomyosin细胞骨架和离子载体之间的相互作用仍然在很大程度上未被探索.
研究的目的:
- 阐明了actomyosin细胞骨在细胞体积调节中的作用.
- 为了研究缓慢的二次体积增加 (SVI) 在低压性休克后的机制.
主要方法:
- 使用了正常类型 (NIH-3T3,MCF-10A) 和癌细胞系 (HT1080,MDA-MB-231).
- 施加了低压冲击和机械拉伸.
- 研究的 (Ca2+) 通过Piezo1通道流入.
- 分析了actomyosin重塑,与NHE1的埃兹林相互作用,以及核变形.
- 进行了转录基因分析.
主要成果:
- 发现了一种新的细胞体积调节系统,通过细胞骨激活离子载体来控制.
- 低压冲击通过Piezo1诱导Ca2+流入,触发了actomyosin重塑和NHE1激活,导致正常细胞中的SVI.
- 在癌细胞中没有SVI,其中体积调节仅依赖于离子载体.
- 通过机械拉伸证明了NHE1的细胞骨激活及其依赖基质合规性的情况.
- 在SVI期间细胞骨激活NHE1,诱导核变形和即时的转录组变化,影响细胞增殖.
结论:
- 亚克托米奥辛细胞骨架通过机械感应在细胞体积调节中发挥着关键作用.
- 和actomyosin介导的信号整合机械线索来调节离子运输,细胞体积,基因表达和增殖.
- 这种机制在癌细胞中受损,突出显示了它在正常生理过程中的重要性.
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