Search research articles

Search research articles

相关实验视频

Updated: Jul 16, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

无脚手架的多细胞3D组织构建利用生物直角点击策略.

Yurui Xu¹, Anwei Zhou², Weiwei Chen¹

¹National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Chemistry and Biomedicine Innovation Center, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing 210093, People's Republic of China.

|September 11, 2023

概括

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Manufacturing and application of high-precision corner cube retro-reflectors using a counterweight separator process.

Applied optics·2026

Same author

Nanopiezoelectric 3D-Bioprinted Neural Organoid Models Epileptic Neuron-Microglia Circuit in Neurodegeneration.

Nano letters·2026

Same author

Low-loss AlN films grown on sapphire by high-temperature MOCVD.

Optics express·2026

Same author

Bioengineered Probiotic-Prebiotic Hierarchical Microspheres With pH-Responsive Architecture Reprogram Immunometabolism in Obesity-Related Disorders.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Bioorthogonal Click-Engineered Microneedle Patch Enables Adeno-Associated Virus-Mediated Dermal Acidic Fibroblast Growth Factor Expression for Hair Loss Treatment.

ACS nano·2026

Same author

Construction of an FSH induced liver organoid model for investigating lipid metabolism abnormalities and study of the regulatory role of key lncRNAs.

Genomics·2026

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

研究人员开发了一种新的生物直角工程策略 (BIEN),以创建先进的多细胞3D组织结构 (MTC). 这种无支架的方法快速组装MTC,改进了体外和体内癌症研究模型.

科学领域:

生物材料工程生物材料工程
组织工程是组织工程.
合成生物学合成生物学

背景情况:

多细胞3D组织结构 (MTC) 对于模仿生物医学研究中的本地组织结构和功能至关重要.
现有的MTC生成方法通常依赖于支架,这可能会带来局限性.
需要先进的,无脚手架的方法来创建更生理相关的MTC.

研究的目的:

引入一个新的生物对角工程策略 (BIEN) 来实现无脚手架的MTC生成.
为了证明BIEN能够创建具有增强细胞间连接和多能性的均异质球体的能力.
评估BIEN生成的MTC在改善体外和体内癌症研究模型中的有效性.

主要方法:

利用细胞生物合成机械,将生物对等的化物记者纳入细胞表面的葡萄糖合物.
采用了亚化物修饰细胞和多臂聚乙烯糖醇 (PEG) 之间的点击反应,用于快速的MTC组装.
描述了由此产生的MTCs的统一性,异质性,细胞间结合和多能性.

主要成果:

通过BIEN策略成功生成了均和异质的球体.
在MTC中实现了高度的细胞间结合,并保持了细胞多能性.
证明BIEN生成的MTC显著改善皮下异种移植模型,增强了体外和体内研究.

关键词:

生物直角工程的战略.在体外和体内生物研究模型的研究模型.多细胞的3D组织构造.生理学架构,生理学建筑.没有脚手架的脚手架.

更多相关视频

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

相关实验视频

Last Updated: Jul 16, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

结论:

生物直角工程策略 (BIEN) 为生成先进的MTC提供了一种变革性的,无脚手架的方法.
BIEN促进了创建有效模拟瘤特征和细胞异质性的MTCs.
这种方法通过提供卓越的体外和体内模型,为推进癌症研究提供了重大前景.