生物信息学分析CUL2/4A/9及其在头部和部状细胞癌中的功能
Bingqing Xu1, Ruohuang Wang1, Jisheng Zhang1
1Department of Otolaryngology-Head and Neck Surgery, Key Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.
Endokrynologia Polska
|September 11, 2023
概括
库林 (CUL) 蛋白CUL2,CUL4A和CUL9在头部和部状细胞癌 (HNSCC) 中表达的变化. 这些CUL蛋白影响HNSCC细胞的增殖,迁移和免疫反应,表明它们作为生物标志物的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 库林 (CUL) 蛋白与癌症信号通路有关.
- 在头部和部状细胞癌 (HNSCC) 中,CUL蛋白的特定作用仍然在很大程度上未被阐明.
研究的目的:
- 研究HNSCC.中CUL家族蛋白的表达模式.
- 探索CUL蛋白表达与HNSCC瘤发生,预后和免疫微环境的相关性.
- 评估CUL蛋白对HNSCC细胞行为的功能影响.
主要方法:
- 利用癌症基因组图集 (TCGA) 和cBioPortal进行大规模数据分析.
- 使用生物信息学工具 (Metascape,STRING,Cytoscape,TIMER,Kaplan-Meier绘图器,TISIDB) 进行表达和相关性分析.
- 通过RT-qPCR验证了mRNA水平,并使用CCK8和伤口愈合试验评估了细胞增殖/迁移.
主要成果:
- 与正常组织相比,在HNSCC中,CUL2和CUL4A的表达受到上调,而CUL9在正常组织中受到下调.
- 生物信息学分析将CUL2/4A/9表达与HNSCC的临床病理特征和整体存活率联系起来.
- 发现CUL2/4A/9可以影响瘤特异性免疫反应,包括瘤透淋巴细胞 (TIL) 和免疫调节剂.
- CUL2/4A/9显著促进了HNSCC.中的细胞增殖和迁移.
结论:
- 在HNSCC中观察到CUL2/4A/9的转录水平发生变化.
- 这些CUL基因在HNSCC细胞增殖和迁移中发挥作用.
- CUL2/4A/9代表了HNSCC患者潜在的新型独立生物标志物.
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