基于里埃变换红外光谱的方法作为Candida parapsilosis临床分离物的新型打字工具
Elena De Carolis1, Brunella Posteraro2,3, Benedetta Falasca2
1Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS , Roma, Italy.
基于里埃变换红外光谱的IR生物型号显示出可能导致血液感染的类型是*Candida parapsilosis*. 虽然有歧视性,但它在跟踪抗可纳菌株方面不如微观卫星类型有效.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 分子类型的定型.
背景情况:
- 由*Candida parapsilosis*引起的鼻腔血流感染 (BSI) 是一个重大问题,特别是随着抗可纳分离物的增加.
- 有效的分子排型方法对于控制*C. parapsilosis*在医院内传播至关重要.
研究的目的:
- 评估基于里埃变换红外光谱法 (FTIR) 的IR生物型对*Candida parapsilosis*分离物的分辨力.
- 为了将IR Biotyper的性能与微卫星 (STR) 类型进行比较,用于分类抗可纳*C. parapsilosis*菌株.
主要方法:
- 通过IR生物型和微卫星 (STR) 排型,分析来自血液感染的59种*C. parapsilosis*分离物 (包括耐富可纳和敏感菌株).
- 进行了IR生物类型分析,使用自动和初步切断值来评估其类型性.
- 基因相关性是根据ERG11*基因 (Y132F和R398I) 和微卫星资料的突变确定的.
主要成果:
- IR生物型确定了与ERG11*突变 (Y132F或Y132F/R398I) 相关联的独特集群.
- 调整截止值提高了IR Biotyper区分分离子的能力,包括分离异组菌株.
- 红外生物型和STR类型之间的一致性从47%到74%不等,STR显示出更高的歧视力.
结论:
- 红外生物型是用于*Candida parapsilosis*类型的歧视性工具,特别是在抗可纳分离物中.
- 微卫星 (STR) 排版仍然是首选的方法,因为它具有更高的区分能力.
- 需要对较大的孤立集合和相关的 *Candida * 种类进行进一步的研究,以充分确定 IR Biotyper 的实用性.
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