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粘附力对croRS基因表达和Enterococcus faecalis抗生素耐药性的影响
Xue Wang1,2, Siyu Wang1,2, Lingling Yuan1,2
1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Journal of biomedical materials research. Part A
|September 11, 2023
概括
材料的表面特性显著影响细菌粘附和抗生素耐药性. 疏水的表面增加了Enterococcus faecalis对 ceftriaxone的抵抗力,通过调节CroRS系统,建议表面修改作为治疗策略.
科学领域:
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 细菌对表面的粘附受材料特性的影响.
- 在Enterococcus faecalis中,双组分系统CroRS与头菌素耐药性有关.
- 生物膜抗生素耐药性是一个重大的临床挑战.
研究的目的:
- 研究材料表面特性对Enterococcus faecalis生物膜形成和抗生素耐药性的影响.
- 为了确定表面的疏水性是否会影响croRS双组件系统的表达.
- 评估表面性质对E. faecalis生物膜中 ceftriaxone耐药性的影响.
主要方法:
- 聚乙烯 (PE) 和不钢 (SS) 的表面疏水性 (水接触角度) 的表征.
- 使用原子力显微镜测量细菌粘附力.
- 通过定量PCR对croRS基因转录水平的量化.
- 使用殖民地形成单元 (CFU) 计数评估 ceftriaxone 的敏感性.
主要成果:
- 与不钢 (SS) (33.5°,3.5nN) 相比,聚乙烯 (PE) 表面具有更高的疏水性 (97.1°) 和更强的E. faecalis粘合力 (7.6nN).
- 与SS表面相比,在PE表面的E. faecalis中观察到更高的croRS基因转录水平.
- 在疏水性PE上的E. faecalis生物膜与在疏水性SS上的生物膜相比,对 ceftriaxone的耐药性增加.
结论:
- 表面特性,特别是疏水性和粘附力,调节了E. faecalis CroRS系统的表达.
- 材料表面特征可以影响细菌对抗生素 (如塞夫松) 的敏感性.
- 修改生物医疗器械的表面特性可能会提高抗微生物治疗对E. faecalis生物膜的疗效.
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