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高DFI精子中的AKAP4/PKA信号通路减少会影响精子电容

Kun Zhang^1,2, Xiu-Hua Xu¹, Jian Wu¹

¹Hebei Key Laboratory of Infertility and Genetics, Department of Reproductive Medicine, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.

Asian journal of andrology

|September 11, 2023

概括

高精子DNA碎片化指数 (DFI) 与IVF-ET中的受精率降低有关. 这是由于AKAP4/PKARII蛋白减少,精子电容受阻,以及高DFI患者中较少的体反应造成的.

科学领域:

生殖生物学生殖生物学
精子细胞的功能是:
在体外受精 (IVF)

背景情况:

精子DNA碎片化指数 (DFI) 是精子DNA完整性的关键指标.
在接受体外受精-胚胎移植 (IVF-ET) 的患者中,DFI升高与生殖结果不佳有关.
激酶定蛋白 (AKAP) 和精子蛋白抗原17 (SPA17) 在精子功能中起作用.

研究的目的:

调查IVF-ET期间高精子DFI (H-DFI) 的患者受精率下降的根本原因.
为了比较H-DFI和低DFI (L-DFI) 患者之间的AKAP,蛋白激酶A调节子单元 (PKARII) 和SPA17的表达水平.
阐明将H2O2诱导的氧化应激,DFI和精子功能受损联系在一起的分子机制.

主要方法:

在H-DFI和L-DFI患者组的精子中对AKAP,PKARII和SPA17表达的比较分析.
在体外诱导精子DFI使用过氧化 (H2O2) 模拟氧化应激.
评估精子电容,体反应,胚胎培养数据和试管婴儿结果.

主要成果:

在H-DFI患者中,SPA17显示异常转录,但翻译增加.
在H-DFI患者中,AKAP4/PKARII蛋白的表达显著下降.
H2O2治疗增加了SPA17蛋白,并降低了AKAP4/PKARII的表达,抑制了精子电容和体反应.

结论:

降低H-DFI患者的受精率是由于精子AKAP4/PKARII蛋白质的损失造成的.
精子容量受损和体反应发生的减少是高DFI的关键后果.
氧化压力导致SPA17和AKAP4/PKARII表达的改变,对试管婴儿的成功产生负面影响.

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