红细胞中CCN2衍生的circRNAs的表达和功能
Soma Kato1,2, Kazumi Kawata1, Takashi Nishida1
1Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-Cho, Kita-Ku, Okayama, 700-8525, Japan.
Journal of cell communication and signaling
|September 11, 2023
概括
来自细胞通信网络因子2 (CCN2) 的新型循环RNAs (circRNAs) 存在于软质细胞中,并影响软质细胞的分化,影响农糖和蛋白质糖的合成.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞通信网络因子2 (CCN2) 对于内分泌骨化和软骨修复至关重要.
- 循环RNAs (circRNAs) 调节基因表达,并且已知在某些细胞类型中来自CCN2.
- 目前尚不清楚冠状细胞中CCN2衍生的circRNA的存在和功能.
研究的目的:
- 为了研究体细胞中CCN2衍生的circRNAs的表达和功能.
- 在人体冠状细胞中识别新型CCN2衍生的circRNAs.
- 阐明这些circRNAs在chondrogenic分化中的作用.
主要方法:
- 用RT-PCR分析检测和放大CCN2衍生的circRNAs在人类冠状细胞HCS-2/8细胞中.
- 核酸测序用于识别新型circRNAs.
- 在冠状细胞分化过程中,在小鼠ATDC5细胞中确认circRNA表达.
- 在hcs-2/8细胞中,siRNA介导的特定ccn2衍生circRNA的淘汰.
主要成果:
- 在HCS-2/8细胞中发现了新型CCN2衍生的circRNA,与以前已知的不同.
- 在ATDC5细胞中证实了几种CCN2衍生的circRNAs的表达,并随着状细胞分化而变化.
- 一个CCN2衍生的circRNA的淘汰导致了aggrecanmRNA表达和proteoglycan合成的显著下降.
结论:
- 由CCN2衍生出的circRNAs在软体细胞中表达.
- 这些circRNAs在chondrogenic分化中起着重要作用.
- 来自CCN2的circRNAs是软骨形成和再生的潜在调节者.
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