大米爆发真菌Pyricularia oryzae的毒性作用因子的适应性进化
Marie Le Naour-Vernet1, Florian Charriat1, Jérôme Gracy2
1PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD, Montpellier, France.
PLoS pathogens
|September 11, 2023
概括
对于大米爆发真菌的毒性至关重要的MAX效应体,显示出由积极选择驱动的高遗传多样性. 这种聚焦在特定结构区域的分子变异可能会影响病原体适应和宿主相互作用.
科学领域:
- 植物病理学 植物病理学
- 菌类基因组学 菌类基因组学
- 分子进化是分子进化的过程.
背景情况:
- 植物病原体利用效应蛋白来操纵宿主过程并引起疾病.
- 结构基因组学揭示了具有保存结构但序列多样化的真菌效应体.
- 马克斯效应物是大米爆发真菌Pyricularia oryzae的关键毒性因素.
研究的目的:
- 在结构相似的MAX效应器中研究驱动序列变化的进化力.
- 描述Pyricularia oryzae中MAX效应者的遗传多样性和选择压力.
主要方法:
- 结构告知基因注释以识别120个隔离物中MAX效应基因.
- 泛基因组分析以评估基因存在/不存在的多态性.
- 基因敲门实验,以评估病毒性贡献.
- 对分子变异和积极选择对效应器序列的作用的分析.
主要成果:
- 每个基因组中确定了58-78个MAX效应基因,存在/缺失差异很大.
- 马克斯效应因子基因表达是宿主依赖的,在早期感染期间达到顶峰.
- 基因损失没有强烈影响病毒性,这表明其他损失驱动因素.
- 高稳定变化和非同义替代率表明强烈的积极选择.
结论:
- 积极选择塑造了MAX效应器的分子多样性,特别是在特定的结构位置.
- 了解MAX效应器多态性对于阐明它们的功能和宿主相互作用机制至关重要.
- 这项研究为未来对植物病原体的效应器进化和毒性研究提供了基础.
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