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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Mass Analyzers: Overview01:13

Mass Analyzers: Overview

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
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相关实验视频

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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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通过DO-MS对DIA质谱的数据驱动优化.

Georg Wallmann1, Andrew Leduc1, Nikolai Slavov1,2

  • 1Departments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, Northeastern University, Boston, Massachusetts 02115, United States.

Journal of proteome research
|September 11, 2023
PubMed
概括

我们开发了DO-MS v2.0,这是一个数据驱动的工具,用于优化质谱 (MS) 方法的数据独立采集 (DIA) 和单细胞蛋白质组学. 该框架提高了大规模蛋白质组研究的蛋白质量测定准确度和数据质量.

关键词:
在DO-MS中,它是DO-MS.在MSMSMSMSMSMSMSMSMSMSMS中,我们可以看到收购 收购 收购 收购控制 控制 控制 控制数据数据的数据数据的数据.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量优化的优化优化优化.在PlexDIA中使用PlexDIA.蛋白质组学 蛋白质组学质量质量质量质量质量质量.单细胞,可视化可视化

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Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
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相关实验视频

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 分析化学 分析化学
  • 生物技术是生物技术.

背景情况:

  • 质谱法 (MS) 对于蛋白质量化至关重要,但对于大型数据集需要优化方法.
  • 数据独立采集 (DIA) 提供高吞吐量和灵敏度,但需要有效的数据采集和质量控制.
  • 单细胞蛋白质组学在数据采集和分析方面提出了独特的挑战.

研究的目的:

  • 介绍DO-MS app v2.0,这是一个针对DIA的MS方法的数据驱动优化框架.
  • 为了实现对无标签DIA,多重DIA (plexDIA) 和单细胞蛋白质组学至关重要的参数的优化.
  • 为大规模蛋白质组数据集提供有效的质量控制和评估工具.

主要方法:

  • 开发DO-MS app v2.0框架,以优化MS数据采集.
  • 框架的应用以优化工作周期,分离和调查扫描参数.
  • 使用DO-MS进行单细胞plexDIA数据的质量控制.
  • 交互式数据可视化和自动报告生成.

主要成果:

  • DO-MS v2.0 促进了对无标签和多重复合 DIA (plexDIA) 的优化.
  • 该框架支持针对单细胞蛋白质组学应用的特定优化.
  • 已证明的使用案例包括优化工作周期,分离和调查扫描号码.
  • 启用了交互式数据显示和生成可共享的质量报告.

结论:

  • DO-MS v2.0 是优化 DIA 质谱法的一种有价值的工具.
  • 该框架提高了蛋白质量化的准确性和大规模蛋白质组学的数据质量.
  • DO-MS v2.0增强了MS在单细胞蛋白质组学研究中的潜力.