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通过使用p-aminobenzoate N-oxygenase从葡萄糖中生产p-Nitrobenzoate:AurF

Ayana Mori¹, Yuuki Hirata¹, Mayumi Kishida¹

¹Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.

Enzyme and microbial technology

|September 11, 2023

概括

研究人员设计大肠杆菌从葡萄糖中产生p-nitrobenzoate (PNBA),通过优化代谢途径和破坏降解基因,实现高产量. 这表明了可持续的微生物平台,用于合成酸芳香化合物.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

酸芳香化合物是重要的工业化学品.
目前的生产方法面临着危险工艺和地区选择性差的挑战.
开发可持续和高效的合成路径至关重要.

研究的目的:

设计一种微生物菌株,从葡萄糖中直接生产p-nitrobenzoate (PNBA).
通过优化代谢途径和最大限度地降低降解来提高PNBA产量.
探索微生物平台对于酸芳香化合物合成的潜力.

主要方法:

通过破坏PNBA降解基因 (nfsA,nfsB,nemA,azR) 来构建大肠杆菌菌株PN-1.
在PN-1中表达的Streptomyces thioluteus AurF用于从p-aminobenzoate中生产PNBA.
通过共同表达paba,pabB,pabC和aurF来设计直接的葡萄糖到PNBA通路.
通过破坏竞争中的代谢途径 (pheA, tyrA, trpE, pykA, pykF) 进一步优化了菌株 (PN-4Ap).

主要成果:

使用PN-1菌株从p-aminobenzoate中获得945mg/L的PNBA产量.
直接从葡萄糖中产生393mg/L的PNBA,使用paba,pabB,pabC和aurF的共同表达.
优化的PN-4Ap菌株在72小时的培养后从葡萄糖中获得了975mg/L的PNBA.
通过代谢工程证明了PNBA标位的显著改善.

结论:

成功开发了一种代谢工程的大肠杆菌菌株,用于高效地从葡萄糖中产生PNBA.
工程微生物平台为传统化学合成提供了一个可持续和潜在的更安全的替代方案.
这项研究强调了微生物合成对各种酸芳香化合物的广泛适用性.

关键词:

在AURF和AURF之间.美国大肠杆菌 (E. coli).微生物生产的微生物生产.这就是N-oxygenase的作用.酸芳香化合物是一种酸芳香化合物.

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