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High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
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在高清电解微电极阵列上优化mRNA转染,可实现98%的效率和多重基因传递

Bastien Duckert1, Dennis Lambrechts2, Dries Braeken2

  • 1Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200d, 3001, Leuven, Belgium; imec, Kapeldreef 75, 3001, Leuven, Belgium.

Biosensors & bioelectronics
|September 11, 2023
PubMed
概括

高分辨率电穿孔 (HD-EP) 使用优化的电脉冲在初级纤维细胞中实现了98%的传染效率. 这种方法使得剂量控制的mRNA输送和空间分辨的顺序转染能够进行大规模查.

关键词:
附着细胞是附着细胞.CMOS微电极阵列中的微电极阵列.设计实验的设计.配送RNA配送RNA的时间空间分辨率的电穿孔.转化 转化 转化 转化

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...