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脂蛋白Eb (On-ApoEb) 能够保护Oreochromis niloticus免受Streptococcus agalactiae感染的影响

Jiamin Feng¹, Yongxiong Huang¹, Meiling Huang¹

¹Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, China.

Fish & shellfish immunology

|September 11, 2023

概括

这项研究确定了尼罗河鱼中的Apolipoprotein E (ApoE),揭示了它在通过减少炎症和改善生存率来对抗细菌感染方面的关键作用.

科学领域:

* 免疫学和鱼类健康
* 分子生物学和生物化学

背景情况:

*Apolipoprotein E (ApoE) 已被公认为其在感染和炎症防御中的作用.
* ApoE对鱼类细菌感染的免疫功能在很大程度上仍未被探索.

研究的目的:

* 克隆和描述来自Oreochromis niloticus的阿波脂蛋白E基因 (On-ApoEb).
*为了研究On-ApoEb在尼罗河鱼中对Streptococcus agalactiae感染的免疫功能.

主要方法:

* 克隆了Oreochromis niloticus的On-ApoEb的全长cDNA.
* 对On-ApoEb序列与其他物种相同性的分析.
* 在细菌感染后的各种组织中对On-ApoEb的表达分析.
*对On-ApoEb对炎症因子,亡,细菌负载和生存率的影响的评估.

主要成果:

* On-ApoEb cDNA 序列为 831bp,编码为 276 氨基酸蛋白质,与鱼类 ApoEb.
* 在Streptococcus agalactiae感染后,肝脏和其他组织上调了On-ApoEb的表达.
* On-ApoEb 已显示出抗炎和抗亡作用,减少了细菌负载,并提高了鱼类的生存率.

结论:

*On-ApoEb在尼罗河鱼对细菌感染的免疫反应中发挥着重要作用.
*On-ApoEb具有抗炎和抗亡性质,有助于宿主防御.
* 这项研究强调了ApoE作为改善鱼类免疫力和抗病能力的潜在目标.

关键词:

在 ApoEEE 中.细胞灭亡 (apoptosis) 是一种死亡的过程.与免疫相关的途径.炎症炎症是一种炎症.色色素尼洛蒂库斯 (Oreochromis niloticus) 是一种多彩的植物.链球菌的 agalactiaeae 是一个细菌.

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