PRRSV改变了甲基化和替代拼接,以调节免疫,细胞外矩阵相关的功能
Chenghong Lin1, Mu Zeng1, Jia Song1
1Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528225, China.
International journal of biological macromolecules
|September 11, 2023
概括
猪生殖和呼吸系统综合征病毒 (PRRSV) 感染改变了基因表达,替代拼接和N6-甲基氨酸 (m6A) 修饰. 这些变化影响关键的细胞通路,可能有助于病毒复制并提供治疗点.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 感染对猪的健康有重大影响.
- 导致PRRSV病变的分子机制,特别是关于宿主基因调节的分子机制,尚未完全理解.
研究的目的:
- 研究PRRSV感染对宿主基因表达,替代拼接和N6-甲基氨酸 (m6A) 修改的影响.
- 为了确定关键的基因和涉及PRRSV病变的途径.
主要方法:
- 采用了转录组测序 (RNA-seq) 和m6A个人核酸分辨率交联和免疫沉降测序 (MeRIP-seq).
- 差异性基因表达,替代拼接事件和m6A修饰部位在感染PRRSV和控制猪肺部中进行了分析.
主要成果:
- 在感染PRRSV的猪中发现了1624个差异表达基因 (DEG).
- 观察到替代拼接 (54,367事件) 和m6A修改 (2265 DASEs) 的显著变化.
- 像LMO7,ZNF185和ECM1这样的基因表现出改变的表达和拼接,影响了参与细胞信号和细胞外矩阵调节的下游通路.
结论:
- 感染PRRSV严重影响宿主细胞基因表达,替代拼接和m6A修饰模式.
- 改变LMO7,ZNF185和ECM1的表达,以及它们的下游目标,可能会促进病毒入侵和复制.
- 这些发现突出了PRRSV感染的潜在治疗点.
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