相关实验视频

Updated: Jul 16, 2025

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Published on: May 13, 2022

基于含化物光探针的精确方法来监测天然水中的基

Yaoyu Xu¹, Jiaying Ding¹, Chengjie Zhang¹

¹Henan Provincial Research Center for Precise Synthesis of Fluorine-Containing Drugs, Anyang Normal University, Anyang, Henan 455000, China.

The Science of the total environment

|September 11, 2023

概括

研究人员开发了一种新的基光探针,用于检测水中的基基 (OH). 这种敏感的探测器可以准确量化自然水域和生物系统中的OH水平.

科学领域:

环境化学环境化学
分析化学分析化学
生物化学生物化学

背景情况:

基 (OH) 是自然水中的关键反应物种,驱动有机和无机化合物的氧化.
OH基的低度和短寿命限制了水生环境中的定量测量研究.

研究的目的:

开发一种新型,灵敏和选择性的光探针,用于在水系统中定量检测基 (OH).
为了证明探头在复杂的生物和环境样本中的适用性.

主要方法:

合成一种新的基光探针,用化物替代的供体.
探测器对基基的反应的光谱表征,包括光增强和斯托克斯转移.
探针在活细胞,斑马鱼和自然水样中检测OH的应用.

主要成果:

开发的探针显示高选择性OH激素与23倍光增强.
探测器表现出114纳米的巨大的斯托克斯转移,促进了信号检测.
在活细胞,斑马鱼和天然水样本中成功检测OH激素,回收率超过92%.

结论:

这种基于的新型探针提供了一种敏感和选择性的方法,用于在各种系统中量化基.
该工具解决了OH激素检测的局限性,使环境和生物背景下进一步研究成为可能.
探测器的低毒性和在各种样本中的适用性突出显示了其在现实世界应用中的潜力.

关键词:

检测检测检测检测光发射探测器进行光.化物的化物.这是一种基基.图像成像是一种成像.天然水是自然的水,自然的水是自然的水.

更多相关视频

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

相关概念视频

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.