通过纳米技术改善基于RNA的作物保护,并从跨王国RNA贩运的见解中获取见解
Angela Chen1, Lida Halilovic1, Jia-Hong Shay1
1Department of Microbiology and Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA, USA.
Current opinion in plant biology
|September 11, 2023
概括
喷雾诱导基因沉默 (SIGS) 使用RNA保护作物,但RNA迅速降解. 纳米颗粒可以保护RNA,改善SIGS,用于生态友好的害虫和病原体控制.
科学领域:
- 农业科学 农业科学
- 生物技术是生物技术.
- 纳米技术 纳米技术
背景情况:
- 喷雾诱导基因沉默 (SIGS) 通过使用外源RNA来沉默病原体/害虫基因,提供环保的作物保护.
- 环境RNA降解限制了SIGS的有效性.
- 大自然在跨王国RNA干扰中利用细胞外囊泡进行RNA传输.
研究的目的:
- 审查纳米技术的进步,以提高RNA稳定性和SIGS中的传递.
- 探索纳米载体设计原则,以有效的基于RNA的作物保护.
- 弥合对RNAi,纳米载体和植物病原体相互作用的理解,以改进SIGS策略.
主要方法:
- 关于RNA稳定性,内化和纳米粒子介导的传递现有文献的审查.
- 对影响RNA保护和吸收的纳米载体属性的分析.
- 综合关于sRNA生物发生,跨王国RNAi和植物病原体相互作用的知识.
主要成果:
- 纳米颗粒在防止RNA降解和促进SIGS的内部化方面表现有前途.
- 已经确定了有效的RNA输送的关键纳米载体属性.
- 了解自然RNAi机制可以为农作物保护提供纳米载体设计的信息.
结论:
- 纳米技术提供了创新的解决方案,以克服SIGS中的RNA不稳定性.
- 工程化纳米纤维可以提高基于RNA的作物保护策略的有效性.
- 进一步的研究将纳米技术和RNAi生物学整合起来,对于可持续农业至关重要.
相关概念视频
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
01:59Plant Breeding and Biotechnology
19.0K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.0K
01:23Types of RNA
63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K
02:30siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
01:29Translational Regulation
42
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
42