一个含有feruloyl esterase的archaeal 盖片降解聚乙烯四甲
Pablo Perez-Garcia1,2, Jennifer Chow1, Elisa Costanzi3
1Department of Microbiology and Biotechnology, University of Hamburg, Hamburg, Germany.
研究人员发现了PET46,一种能够降解聚乙烯四甲酸盐 (PET) 塑料的古老酶. 这一发现扩大了已知的塑料降解酶的多样性,包括古生物,为生物修复提供了新的可能性.
科学领域:
- 生物化学 生物化学
- 环境微生物学 环境微生物学
- 结构生物学 结构生物学
背景情况:
- 聚乙烯二甲 (PET) 是一种普遍存在的环境污染物.
- 许多细菌和真菌酶 (PETases) 可以降解PET,但以前没有发现过任何古老酶.
- 已知的PET降解酶来源于有限的微生物类.
研究的目的:
- 为了识别和描述具有PET降解能力的新型考古酶.
- 扩大已知的能够去聚合聚乙烯四甲酸盐的酶的多样性.
- 为了研究一个新发现的考古PET降解酶的结构和生化特性.
主要方法:
- 基于序列的元基因组搜索以确定潜在的酶.
- 通过X射线晶体学 (1.71 Å分辨率) 进行结构性表征.
- 生物化学试验用于评估PET及其衍生物 (BHET,MHET) 的降解活性.
主要成果:
- 发现和描述PET46,一种来自Candidatus Bathyarchaeota的考古性散乱型烯酸乙酶.
- 在半晶体PET粉末上,PET46表现出显著的降解活性,与已知的细菌酶 (IsPETase,LCC) 相当.
- 在PET46中,对双二乙烯基甲酸盐 (BHET) 和单二乙烯基甲酸盐 (MHET) 的活性较高.
- 结构分析显示了一种保存的α/β-酶折叠,具有独特的盖盖结构,参与基质结合.
- PET46是一种适应热的酶,这表明它具有工业应用的潜力.
结论:
- 这项研究确定了第一个能够降解聚乙烯四甲酸盐 (PET) 的古代酶,PET46.
- 这一发现扩大了PET-化酶的遗传学多样性.
- 植物细胞壁降解酶PET46显示出显著的PET脱聚合活性,为塑料生物修复策略提供了新的途径.
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