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相关概念视频

Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

2.6K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
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Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.4K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.4K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Porin Insertion in the Outer Mitochondrial Membrane01:12

Porin Insertion in the Outer Mitochondrial Membrane

3.1K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
3.1K
Structure of Porins01:21

Structure of Porins

3.0K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
3.0K
Mitochondrial Membranes01:45

Mitochondrial Membranes

11.5K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
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相关实验视频

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Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
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普雷福丁2有助于线粒体形态和功能.

Ismail Tahmaz1, Somayeh Shahmoradi Ghahe1, Monika Stasiak1

  • 1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106, Warsaw, Poland.

BMC biology
|September 12, 2023
PubMed
概括
此摘要是机器生成的。

预foldin子单元,特别是pfd2,对于酵母细胞在热和呼吸应激下生存至关重要. 这种细胞质的辅助器支持线粒体功能和蛋白质平衡,特别是在细胞应激期间.

关键词:
陪伴者是指一个陪伴者.线粒体中的线粒体.在Pfd2/Gim4中使用.在Prefoldin中进行预折叠.蛋白质稳定性 蛋白质稳定性姆7070的时间

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Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
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科学领域:

  • 细胞生物学 细胞生物学
  • 蛋白质平衡是蛋白质的平衡.
  • 线粒体生物学 线粒体生物学

背景情况:

  • 普雷福丁是TRiC/CCT复合体的一个进化保守的共同主管,参与折叠新合成的细胞骨蛋白质.
  • 它的功能延伸到维持蛋白质平衡,特别是在细胞应激条件下,如线粒体功能障碍.
  • 预foldin独立于ATP运作,使其适合应激环境.

研究的目的:

  • 调查前丁子单元在Saccharomyces cerevisiae细胞增殖和在各种压力条件下的生存中的作用.
  • 为了确定prefoldin在热和呼吸应激期间对细胞反应的特定贡献.
  • 为了阐明前丁和线粒体功能之间的联系.

主要方法:

  • 在Saccharomyces cerevisiae中细胞增殖和存活的分析,缺少在压力下的前折叠子单元.
  • 评估线粒体形态和呼吸链复杂的丰度.
  • 研究了前丁子单元的相互作用,特别是Pfd2与Tom70.0.

主要成果:

  • 在热应激下,prefoldin子单元增强细胞生长.
  • 在呼吸条件下,Prefoldin促进细胞生长.
  • 前折蛋白子单元Pfd2支持线粒体形态,呼吸链复合体,并与线粒体进口受体Tom70.0相互作用.

结论:

  • 细胞质前复合物与线粒体功能有关.
  • 失去Pfd2会触发适应性蛋白质水平反应,这表明它在细胞平衡中持续发挥作用.
  • Pfd2可以通过细胞质量控制直接支持线粒体功能,也可以通过蛋白质平衡网络间接支持线粒体功能.