用 Thermoanaerobacterium AK17 的代谢工程来增加海藻水解剂中的乙醇生产
Antoine Moenaert1,2, Bryndís Bjornsdottir3, Einar Baldvin Haraldsson3,4
1Department of Biotechnology, Matís Ohf, Reykjavík, Iceland. antoine.moenaert@gmail.com.
Biotechnology for biofuels and bioproducts
|September 12, 2023
概括
工程细菌Thermoanaerobacterium AK17 (AK17) 现在可以有效地从海藻糖中产生乙醇. 改进的AK17_M6菌株实现了近乎理论的产量,将可持续生物燃料的产量增加了1.5倍.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物发酵 微生物发酵
背景情况:
- 可再生生物质,特别是海藻,为生物基产品提供了化石燃料的可持续替代品.
- 海藻含有丰富的碳水化合物,适用于生物炼油应用.
- Thermoanaerobacterium AK17是一种本地细菌,能够将各种糖分发酵成乙醇,乙酸盐和乳酸盐.
研究的目的:
- 为了改造Thermoanaerobacterium AK17以提高乙醇的生产.
- 为了研究代谢途径,负责副产品的形成.
- 为了评估该菌株在发酵海藻衍生水解盐中的性能.
主要方法:
- 在Thermoanaerobacterium AK17的基因工程中,通过敲除乳酸和酸盐路径.
- 通过Butyrate路径的招募,进一步的代谢工程来消除乙酸盐生产.
- 使用棕色海藻 (Laminaria digitata) 水解剂进行发酵试验.
主要成果:
- 改造的AK17_M6菌株实现了理论上最大乙醇产量的90%,比野生类型增加了1.5倍.
- 该菌株成功发酵了海藻水解剂,产生了0.45g/g的基质.
- 识别并克服了对乙酸生产的代谢逆转.
结论:
- 工程菌株AK17_M6在从海藻生物质中生产乙醇方面表现出高效率.
- AK17_M6具有广泛的碳水化合物利用概况,包括海藻特有的糖.
- 这种菌株是工业规模第二代和第三代生物燃料生产的有希望的候选人.
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