针对真菌未折叠蛋白质反应的天然产品作为替代作物保护战略
Thomas Charpentier1,2, Guillaume Viault1, Anne-Marie Le Ray1
1Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France.
Journal of agricultural and food chemistry
|September 12, 2023
概括
针对真菌展开蛋白质反应 (UPR) 途径的新天然化合物增强了植物的防御能力. 这项研究确定了克桑作为有前途的IRE1抑制剂,减少了作物疾病的严重程度.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 农作物保护面临着杀菌剂耐药性,气候变化和生态毒性等挑战.
- 准真菌未折叠蛋白反应 (UPR) 是植物疾病控制的一个有希望的策略.
- UPR途径影响真菌的毒性和对植物防御化合物的敏感性.
研究的目的:
- 为了确定抑制真菌IRE1蛋白的天然产品,这是UPR途径的关键作用因子.
- 评估这些化合物的有效性,以增加真菌对植物防御的敏感性.
- 为了研究抑制剂与IRE1结合部位之间的相互作用.
主要方法:
- 开发一种基于细胞的体外查试验,用于IRE1抑制剂.
- 潜在的抑制剂的识别和表征,重点是聚化化化化色素.
- 通过HAC1mRNA拼接试验和分子对接研究验证IRE1抑制.
- 评估活性化合物对Brassica oleracea植物疾病症状的影响.
主要成果:
- 确定了7种潜在的IRE1抑制剂,重点是克桑.
- 化合物γ-曼戈斯表明显著抑制了HAC1mRNA拼接.
- 分子对接揭示了ksanthones和IRE1结合部位之间的详细相互作用.
- 活性桑的亚毒剂量减少了Brassica oleracea感染Alternaria brassicicola和Botrytis cinerea的叶子中的亡.
结论:
- 聚基化前化桑是真菌IRE1蛋白的有效抑制剂.
- 用天然产品针对UPR途径提供了一种新的作物保护策略.
- 这些发现为开发新的环保杀菌剂提供了基础.
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