相关实验视频

Updated: Jul 16, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

在ART中使用的人类卵细胞的转录组完整性:技术和内在因素的影响

Bastien Ducreux¹, Catherine Patrat^2,3, Jacquetta Trasler^4,5,6

¹Université Bourgogne Franche-Comtés-Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, Dijon, France.

Human reproduction update

|September 12, 2023

概括

辅助生殖技术 (ART) 可以影响卵细胞的转录质量. 卵巢刺激,冷保存和生活方式变化等因素可以改变基因表达,可能影响ART的成功和胚胎发育.

科学领域:

生殖生物学和遗传学生殖生物学和遗传学
基因组学和表观遗传学
辅助生殖技术 (ARTs) 是一种辅助生殖技术.

背景情况:

辅助生殖技术 (ARTs) 的安全性尚未完全确立,尽管全球有数以百万计的分娩.
卵细胞质量,而不是胚胎质量,是发育潜力的首要决定因素.
卵巢刺激,冷保存和体外成熟 (IVM) 等ART程序可能会破坏卵细胞的基因组表达.

研究的目的:

审查由于ART干预而导致的人类卵细胞的转录基因变化.
调查内在因素 (年龄,不孕症,生活方式) 对卵细胞转录组学的影响.
为ART程序提出最佳实践,以减轻负面影响.

主要方法:

在PubMed.com上进行了全面的文献搜索.
确定了专注于人类卵细胞转录组的研究.
分析包括来自ART干预的卵细胞,母亲的衰老,生活方式因素和生殖健康问题.

主要成果:

癌症治疗,母亲的年龄,生活方式 (吸烟,BMI) 和不孕 (子宫内膜异位症,PCOS) 都会对卵细胞的转录完整性产生负面影响.
卵巢刺激,IVM和冷保存可以导致参与线粒体功能,新陈代谢,细胞周期和表观遗传调节的基因失调.
表观遗传调节者 (DNA甲基化,基因组修饰) 特别容易受到破坏,可能解释全球转录组变化.

结论:

人工智能治疗程序和外部因素可能会损害卵细胞的转录组完整性,对胚胎发育构成风险.
调整ART协议并尽量减少不良暴露可能会减少这些转录基因失调.
需要进一步的研究,以充分理解和减轻ART对卵细胞质量的影响.

关键词:

在这个过程中,IVF IVF.在RNA-seqqq.辅助生殖技术是一种辅助生殖技术.人类卵子细胞转录组 (transcriptome) 是一个转录组.

更多相关视频

Human Egg Maturity Assessment and Its Clinical Application

Human Egg Maturity Assessment and Its Clinical Application

Published on: August 19, 2019

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

相关概念视频

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...