在水牛中全基因组识别和FGF基因家族的进化分析
Faiz-Ul Hassan1, Tingxian Deng2, Muhammad Saif-Ur Rehman1
1Faculty of Animal Husbandry, Institute of Animal and Dairy Sciences, University of Agriculture, Faisalabad, Pakistan.
Journal of biomolecular structure & dynamics
|September 12, 2023
概括
这项研究在水牛中确定了22个纤维细胞生长因子 (FGF) 基因,揭示了保存的进化模式和独特的突变. 这些发现提供了对水牛遗传学和改善动物表现的潜在选择的见解.
科学领域:
- 基因组学和生物信息学
- 哺乳动物的比较遗传学
- 分子进化分子进化
背景情况:
- 纤维细胞生长因子 (FGF) 是关键的多生长因子,调节哺乳动物的发育,包括细胞分化,增殖和迁移.
- 了解FGF基因家族的特征和进化轨迹对于解读它在水牛等牲畜物种中的作用至关重要.
研究的目的:
- 使用in silico分析,对水牛中的FGF基因家族进行全基因组识别和表征.
- 与相关物种相比,研究水牛FGF基因的物理化学特性,进化模式和保护.
主要方法:
- 从NCBI获得了水牛,牛,山羊和绵羊的基因组和蛋白质组序列.
- 使用ProtParam进行物理化学性质分析,TBtools用于基因结构和基因动机分析,ClustalW用于比较氨基酸分析.
- 进行了遗传学分析,突变分析和重复事件分析.
主要成果:
- 在水牛中确定了22个具有显著物理化学特性 (不稳定性,热稳定性,水友性,基本性质) 的FGF基因.
- 遗传学分析将这些基因分为九个子家族,揭示了跨物种的显著保护.
- 在水牛FGF4,FGF6和FGF19中发现了三种非同义突变;在净化选择中确定了一个细分重复 (FGF10/FGF22).
结论:
- 在水牛中,FGF基因家族在整个进化过程中表现出显著的保护.
- 在水牛FGF基因中发现的非同义突变可能会对旨在提高动物表现的选择性育种计划产生影响.
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