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皮埃佐通道在拉伸效应的人类呼吸道平滑肌肉的发展

Brian Kelley¹, Emily Y Zhang¹, Latifa Khalfaoui¹

¹Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, Rochester, Minnesota, United States.

American journal of physiology. Lung cellular and molecular physiology

|September 12, 2023

概括

在早产婴儿的机械敏感的皮埃佐管道.

科学领域:

肺部医学肺部医学
细胞生物学细胞生物学
新生儿科学新生儿科学

背景情况:

呼吸支,如持续正气道压力 (CPAP),可以过度延伸早产婴儿的气道.
这种机械压力可能导致长期的气道过度反应和重塑,但潜在的机制尚不清楚.
气道光滑肌细胞对于气道收缩和重塑至关重要.

研究的目的:

调查机械敏感的Piezo (PZ) 通道在发展中的气道中对有害的气道应对伸展的作用.
为了确定PZ通道是否在人类胎儿呼吸道光滑肌肉 (fASM) 细胞中表达和功能.

主要方法:

使用了怀孕18-22周的人类胎儿呼吸道光滑肌肉 (fASM) 细胞作为体外模型.
在不同的拉伸条件下评估PZ1和PZ2通道的表达.
在PZ通道激活 (Yoda1或拉伸) 后测量了细胞内 ([Ca2+]i) 反应和细胞外矩阵 (ECM) 生产.

主要成果:

PZ1和PZ2通道在发育的气道光滑肌中表达,它们的表达通过机械拉伸来调节.
通过Yoda1或伸展激活PZ通道显著增加了细胞内水平.
伸展诱导的PZ通道激活导致细胞外基质生产的增加.

结论:

功能性的皮埃佐通道存在于正在发育的气道光滑肌肉中.
这些通道有助于细胞内信号传递和细胞外矩阵重塑,以应对机械拉伸.
研究结果表明,Piezo通道可能会在接受呼吸辅助的早产婴儿中调解有害的呼吸道变化,可能会导致慢性呼吸道疾病.

关键词:

喘喘是一种收缩性收缩性的细胞外矩阵是细胞外矩阵.肺肺肺肺肺肺肺肺肺过早分娩过早分娩是什么

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