手持式交叉极化显微镜用于在人体皮肤中的个体有色素细胞的 in vivo 成像
Rafael Romero1, Jingwei Zhao2, Delaney Stratton3
1Department of Biomedical Engineering, University of Arizona, Tucson, Arizona.
Journal of microscopy
|September 12, 2023
概括
一种新的手持式交叉偏振显微镜 (CPM) 在体内对有色素的皮肤细胞进行成像. 这种创新的设备可视化了黑色素分布和细胞结构,为皮肤色素提供了详细的见解.
科学领域:
- 生物医学光学 生物医学光学
- 皮肤病学 皮肤病学
- 显微镜的使用方法
背景情况:
- 皮肤表面的镜面反射阻碍了地下成像.
- 皮肤中的黑色素颜料吸收特定的光波长,产生对比度.
- 在皮肤病诊断中,体内有色素细胞的高分辨率成像是至关重要的.
研究的目的:
- 开发一种简单的,手持式交叉偏振显微镜 (CPM),用于体内皮肤成像.
- 为了证明CPM在可视化个体色素细胞及其结构方面的能力.
- 评估CPM在揭示皮肤内的颜料三维分布方面的潜力.
主要方法:
- 使用交叉极化检测方法来最大限度地减少表面反射,并增强多重散射光的检测.
- 采用水浸式镜头镜头,具有高数值光圈,用于高分辨率成像.
- 在体内进行了一项试点研究,对皮肤病变进行成像,并与反射共聚焦显微镜共同记录图像.
主要成果:
- 在活体中,CPM成功地对人体皮肤中的个体有色素细胞进行了成像.
- 该装置揭示了有色素细胞和细胞内色素的三维分布.
- CPM图像与反射共聚焦显微镜相对应得很好,识别了含有黑色素的细胞.
结论:
- 开发的手持式CPM是有效的皮肤颜色细胞的体内成像.
- 交叉极化技术允许可视化色素分布和细胞细节.
- CPM为皮肤学研究和诊断提供了一个有前途的工具.
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